种植体周围炎龈沟液中IL-2、IFN-γ、IL-4、IL-6的表达及临床意义

IL-2,IFN-γ,IL-4,IL-6 Expression in Peri-implant Gingival Crevicular Fluid and Clinical Significance

目的探讨探讨白细胞介素2、4、6（IL-2、IL-4、IL-6）、干扰素γ（IFN-γ）在种植体周围炎龈沟液（GCF）中的表达及意义。方法选择2011年6月～2014年6月50例种植体周围炎患者为研究对象。另选择健康种植体患者50例和健康人群50例为对照组。统计3组探诊深度（PD）、龈沟出血指数（SBI）、IL-2、IFN-γ、IL-4、IL-6水平。结果种植体周围炎组PD、SBI、GCF均高于健康种植体组和对照组,差异有统计学意义（P〈0.01）;但健康种植体组和对照组PD、SBI、GCF差异无统计学意义（P〉0.05）;种植体周围炎组IL-2、IFN-γ水平低于健康种植体组和对照组（P〈0.01）,健康种植体组IL-2、IFN-γ水平低于对照组（P〈0.05）,种植体周围炎组IL-4、IL-6水平高于健康种植体组和对照组（P〈0.01）,健康种植体组IL-4、IL-6水平高于对照组（P〈0.05）; ≥60岁组IL-2、IFN-γ水平低于〈60岁组（P〈0.01）, ≥60岁组IL-4、IL-6水平高于〈60岁组（P〈0.01）;种植体周围炎患者GCF中IL-2与IFN-γ呈正相关（r=4.267, P=0.016）,与IL-4、IL-6分别呈负相关（r=4.352、4.615,P=0.005、0.002）,IFN-γ与IL-4、IL-6分别呈负相关（r=4.322、4.603,P=0.009、0.005）,IL-4,IL-6呈正相关（r=4.065, P=0.019）。结论种植体周围炎患者IL-4、IL-6高表达,IL-2、IFN-γ低表达,IL-2、IFN-γ、IL-4、IL-6的表达与种植体周围炎患者性别无关,与种植体周围炎患者的年龄有关。

Objective To study IL-2、IFN-γ、IL-4、IL-6 expression in peri-implantitis gingival crevicular fluid and their clinical significance. Methods Between June 2011 and June 2014, 50 patients with peri-implantitis were selected as the research object. 50 cases of healthy implant patients and 50 cases of healthy people healthy were selected as the controls. The probing depth （PD）, sulcus bleeding index （SBI）, GCF IL-2,IFN-γ,IL-4,IL-6 mass concentration in the three groups were tested. Results PD and SBI, GCF in peri-implantitis group were higher than healthy implant group and the control group, and the difference was statistically significant （P〈0.01）. But PD and SBI, GCF of the healthy implant group and the control group had no obvious difference （P〉0.05）. IL-2, IFN-gamma levels in peri-implantitis group were lower than healthy implant group and the control group （P〈0.01）. IL-2 and IFN-gamma levels in healthy implant group were lower than the control group （P〈0.05）. IL-4, IL-6 levels in peri-implantitis group were higher than healthy implant group and the control group （P〈0.01） and IL-4, IL-6 levels in healthy implant group were higher than the control group （P〈0.05）. IL-2, IFN-gamma levels in ≥ 60 year were higher than 〈 60 year-old group （P〈0.01）. IL-4, IL-6 in ≥ 60 year were lower than 〈60 year-old group（P〈0.01）. IL-2 in GCF in in peri-implantitis group was positively correlated with IFN-gamma （r=4.267, P=4.267）, and IL-4, IL-6 were negatively correlated （r=4.352, 4.615; P=0.005, 0.002）, IFN-gamma, IL-6 and IL-4 were negatively correlated （r=4.322, 4.603; P=0.009, 0.005）, IL-4 and IL-6 were positively correlated （r=4.065, P=4.065）. Conclusion IL-4, IL-6 of peri-implantitis patients have high expression, and IL-2, IFN-gamma of peri-implantitis patients have low expression. The expression of IL-2, IFN-gamma, IL-4, IL-6 have nothing to do with sex of peri-implantitis patients, and associated with inflammation patient＇s age of of peri-implantitis.