HPLC-ELSD法测定香菊片中黄芪甲苷的含量

Determination of the Content of Astragaloside IV from Xiangju tablets by HPLC-ELSD

[目的]建立了采用蒸发光散射检测器测定香菊片中黄芪甲苷含量的高效液相色谱法。[方法]色谱柱采用十八烷基硅烷键合硅胶为填充剂（250 mm×4.6 mm,5μm）。流动相为乙腈∶水（32∶68）,蒸发光散射检测器。[结果]以峰面积积分值的常用对数（X）对进样量的常用对数（Y）线性回归方程为：Y=1.334 6X＋5.929 3（r=0.997 7）,线性范围为0.516~10.320μg,回收率为91.0%,RSD为1.8%。[结论]该方法操作简便、测定结果准确、重复性好,可用于香菊片中黄芪甲苷的含量测定。

[Objective] The research aimed to establish the high performance liquid chromatography determing the content of astragaloside IV from Xiangju tablets by evaporative light-scattering detector.[Method]Chromatographic column with eighteen alkyl silane bonded silica as filler（ 250 mm × 4. 6 mm,5 μm）. Mobile phase was acetonitrile： water（ 32∶ 68）,evaporative light scattering detector.[Result]With the common logarithm of peak area integral value（ X） and the common logarithm of sample volume（ Y） linear regression,the regression equation was Y = 1. 334 6X ＋5. 929 3（ r = 0. 997 7）,linear range was 0. 516-10. 320 μg. The rate of recovery was 91%,RSD was 1. 8%.[Conclusion]The method was simple and reproducible,the determination result was accurate,which can be used for determination the content of Astragaloside IV from Xiangju tablets.