凤仙花中槲皮素和山奈酚的薄层色谱鉴别

Identification of Quercetin and Kaempferol in Impatiens balsamina L. by TLC

[目的]建立凤仙花药材中槲皮素、山奈酚的提取方法,对凤仙花药材中槲皮素、山奈酚进行薄层鉴别。[方法]采用羧甲基纤维素钠硅胶G板分离凤仙花药材中槲皮素、山奈酚成分。[结果]供试品色谱在与对照品色谱相应位置上显相同颜色的斑点,可用于鉴别凤仙花中的槲皮素和山奈酚。[结论]薄层色谱方法简便、专属性强、重现性好,可用于凤仙花药材的鉴别。

[Objective] To develop a specific method for the identification of quercetin and kaempferol in Impatiens balsamina L. by using thinlayer chromato-graphy（ TLC）. [Method]Quercetin and kaempferol were identified using plates of CMC-Na silica gel G. [Result]In the chromatography of the sample,the same-colored speckles appeared at the corresponding position of the reference compounds. [Conclusion] TLC is simple,good reproducibility and specificity. It can be used for the identification of Impatiens balsamina L.