僵蚕中总黄酮提取工艺研究

Optimization of Extraction Technology of Total Flavonoids from Bombyx batryticatus

[目的]优化僵蚕中总黄酮的提取工艺,测定僵蚕中总黄酮的含量。[方法]以总黄酮含量为评价指标,采用单因素和Box-Behnken试验设计,考察乙醇体积分数、提取时间、料液比、提取次数、药材粒径对僵蚕总黄酮提取效果的影响。[结果]僵蚕在体积分数90%乙醇溶液、料液比1∶15(g/ml)的条件下提取1 h的总黄酮得率最高,为1.85%。[结论]优选的总黄酮提取工艺稳定可行,建立的含量测定方法准确可靠。

[Objective] The research aimed to optimize the extraction technology of total flavonoids from Bombyx batryticatus and determine the of total flavonoids.[Method]With total flavonoids content as evaluation index,ethanol volume,extracting time,material ratio,extracting times and the material＇s size were selected to evaluate the effect on the total flavonoids from Bombyx batryticatus by single factor test and Box-Behnken design.[Result]The highest yield of total flavonoids was achieved to 1. 85%,in which the ethanol volume was 90%,material ratio was 1∶ 15（ g / ml）and extracting time was 1h.[Conclusion]The optimum extraction technology of total flavonoids from Bombyx batryticatus was stable and feasible.The detetmination method of total flavonoids was accurate and reliable.