响应面法优化超声波辅助提取泽泻挥发油工艺

Optimization of Ultrasound-assisted Extraction Techniques of Volatile Oil from Alismatis Rhizoma by Response Surface Method

[目的]响应面法优化泽泻挥发油超声波辅助法提取工艺。[方法]以泽泻挥发油得率为指标,在单因素试验的基础上,选取料液比、提取温度和超声时间3个因素进行Box-Benhnken响应面法试验设计,对其提取工艺参数进行优化。[结果]通过软件模型拟优化后得到超声波提取泽泻挥发油的最佳工艺参数为料液比1∶8.27、提取时间41.33 min、提取温度51.4℃,泽泻挥发油得率为6.315%,与理论值较为接近。[结论]响应面法建立的泽泻挥发油提取工艺模型得率高,并能很好地预测试验结果。

[Objective] The research aimed to optimize the ultrasonic-assisted extraction techniques of volatile oil from Alismatis Rhizoma by response surface method. [Method]With yield of volatil oil from Alismatis Rhizoma as index,on the basis of single-factor experiments,the effects of ultrasonic time,solid-liquid ratio and extraction temperature on the extraction yield of volatile oil from Alismatis Rhizoma was investigated with a three-factor Box-Benhnken design. [Result]The optimum extraction parameters were： solid-liquid ratio of 1∶ 8. 27,ultrasonic time41. 33 min,extraction temperature 51. 4 ℃. Under the optimum conditions,the extration rate of volatile oil from Alismatis Rhizoma reached to6. 315%,which was in consistency with the theoretic value. [Conclusion]Ultrasonic-assissted model of volatile oil from Alismatis Rhizoma established by Box-Benhnken design could precisely predict the experiment results well.