雷州黑鸭PID1基因部分序列的克隆与蛋白功能分析

Analysis of Cloning and Protein Function of Partial PID1 Gene Sequence of Leizhou Black Duck

[目的]了解雷州黑鸭磷酸酪氨酸互作结构域1(phosphotyrosine interaction domain containing 1,PID 1)基因的序列结构特征及其蛋白的功能,为利用其改善雷州黑鸭肉品质与提高肉风味提供分子理论依据。[方法]采用RT-PCR方法克隆雷州黑鸭PID1基因部分序列,测序后比对其同源性,构建系统发育树,并预测其蛋白结构与功能。[结果]该核苷酸序列长度为369 bp,编码80个氨基酸,且大多为疏水性氨基酸。与人、牛、鸡等物种进行同源性比对,发现雷州黑鸭PID1基因序列与鸡具有高度同源性,同源性高达94%,且雷州黑鸭与鸡聚于同一系统发育支。蛋白质结构预测表明,该蛋白无跨膜结构与信号肽,氨基酸序列呈疏水性,位于细胞质中。[结论]雷州黑鸭的PID1基因与鸡的亲缘关系最近,其蛋白主要功能是在细胞质中参与肌内脂肪沉积的调控。

[Objective]To understand structure characteristics and protein function of partial PID1 gene sequence of Leizhou black duck,which will provide the molecular basis for improvement of Leizhou black duck and improve the quality of meat flavor. [Method]Using RT-PCR cloning gene partial sequence of the Leizhou black duck PID1,homology sequenced,phylogenetic tree was constructed,and their protein structure and function was forecasted. [Result]A length of 369 bp nucleotide sequence was obtained,the sequence encoding the 80 amino acids,most of which are hydrophobic amino acids. It was found homology alignment and phylogenetic tree species such as people,cattle and chickens,Leizhou black ducks and chickens homology of 94%,together in a phylogenetic tree on. And its protein structure prediction,and found that there is no similar transmembrane signal peptide,and present in the cytoplasm. [Conclusion]Leizhou black duck PID1 protein is involved in the regulation of intramuscular fat deposition in the cytoplasm,and recent genetic relationship with chicken.