2010～2014年小麦遗传转化文献分析

Analysis on the Literature of Wheat Transgenic Studies during 2010-2014

自从1992年第一株转基因小麦诞生以来,小麦的转基因技术经有了很大的发展。该研究分析了Web of knowledge数据库和CNKI数据库收录的2010~2014年发表的关于普通小麦转基因的文献。筛选得到102篇英文文献和103篇中文文献,涉及296个试验,共对152个普通小麦品种进行了遗传转化研究。目前小麦遗传转化主要采用基因枪和农杆菌介导法,分别占总试验数的68.25%和30.4%;少数试验使用花粉管通道和电转化进行转基因。转化靶标组织主要是幼胚及其愈伤组织,占试验总数的80.35%;其次为成熟胚来源的愈伤组织,占8.62%。Bar、HPT、NPTII、PMI和At MYB12分别在不同的试验中被使用,但Bar是最常用的筛选标记,占试验总数的61.22%。不同试验的转化率相差较大,从0.1%到45%不等。

Since the first report of wheat transgenic plants in 1992,significant progress has been made in wheat transformation technology.The paper reporting wheat transgenic studies in Web of knowledge and CNKI published during 2010-2014 were analyzed. 296 experiments and 152 wheat varieties were used in 103 English papers and 103 Chinese papers. Analysis showed that the particle bombardment and the agrobacterium-mediated transformation remain to be the two major approaches for wheat transformation,representing 68. 25% and 30. 4% of all experiment reported,in few experiments pollen tube and electroporation were used to transform wheat. Immature embryo and derived callus were used as recipient tissue in the most studies. Bar,Hpt,Npt II,PMI and At MYB12 were used in different experiments as selection or report genes. The most commonly（ 61. 22%） used selection gene is bar. As for the transformation efficiency reported in wheat,a big variation existed in different experiments,ranging from 0. 1%-45%.