升血小板胶囊联合泼尼松对原发免疫性血小板减少症患者Treg细胞和Th17细胞的影响

Effect of L Platelet Capsule Combined with Prednison on Treg Cells and Th17 Cells of Primary Immune Thrombocytopenia

目的探讨升血小板胶囊联合泼尼松对原发免疫性血小板减少症(ITP)患者外周血CD4^+CD25^+调节性T(Treg)细胞和Th17细胞计数及其分泌因子的影响。方法选取医院收治的免疫性血小板减少性紫癜患者60例,随机以分为两组;实验组给予升血小板胶囊和泼尼松,对照组仅给予泼尼松。对比临床疗效;以流式细胞法测定治疗前后患者空腹静脉血中Treg和Th17细胞数量;以ELISA试剂盒测定血清中细胞因子IL-10和IL-17含量。结果实验组总有效率高于对照组;无论实验组还是对照组患者外周血Treg和Th17细胞数量,以及IL-10含量均显著高于治疗前(P<0.05);实验组Treg细胞数量和IL-10血清含量显著高于对照组。结论升血小板胶囊联合泼尼松治疗可能通过改变Treg细胞数量和调节其功能发挥治疗ITP作用,进一步证实了Treg和Th17细胞数量异常或功能缺陷可能参与了ITP的发生发展。

Objective To investegate the effect of treatment with L platelet capsule prednison on quantity and function of CD4~＋CD25~＋regulatory T（ Treg） and Th17 cells in primary immune thrombocytopenia（ ITP） patients. Methods There were 60 patients were enrolled with requirement of the classic diagnostic criteria for ITP. Patients are divided by randomized method into two groups. The experiment group was concurrently given with L platelet capsule and prednison,while the control group was solely given with prednison. We observe the clinical effects between the two groups. Numbers of Treg and Th17 cells were counted by flow cytometer,while cytokines IL-10 and IL-17 were detected with ELISA kit. Results The experiment group had better clinical effects than the control group（ P〈0. 05）. IL-10 cytokine expression level and the number of Treg and Th17 cell in any group were significantly higher than those before treatment in any groups（ P〈0. 05）. IL-10 level and the number of Treg cell in experiment group were significantly higher than those in the control group（ P〈0. 05）. Conclusion The cotreatment with L platelet capsule and prednison altered the quantity and secretory function of the Treg and Th17 cell,and then improved the clinical effect. These might further confirm that abnormal quantity and function defect of Treg and Th17 cell might be participated in the occurrence and development of ITP.