细胞自噬在多发性创伤大鼠肺组织内的变化

Autophagic changes in lung tissues of rats with multiple trauma

目的观察多发伤性创伤大鼠肺组织内的变化,探讨多发性创伤后急性肺损伤的相关机制。方法选取健康雌性SD大鼠56只,随机分为假手术组(只在颅骨钻孔,28只)和模型组(股骨干闭合骨折合并脑损伤,28只)。分别在术后6 h、12 h、24 h、48 h及5 d取大鼠右肺下叶组织,Real Time-PCR检测各个时间点Beclin-1和LC3-Ⅱ基因的动态表达。结果在以上各个时间点,模型组Beclin-1、LC3-Ⅱ的m RNA水平较假手术组均明显上调。术后48 h模型组Beclin-1、LC3-Ⅱ的m RNA水平(7.532±0.03,10.843±0.03)明显高于假手术组(1.063±0.10,1.091±0.11),差异有统计学意义(P<0.01)。结论自噬可能参与了股骨干骨折合并脑损伤这类严重多发伤后的急性肺损伤。

Objective To investigate the changes of autophagy in lung tissues of rats with mutiple trauma. Methods Fifty-six SpragueDawley（SD） rats were randomly divided into sham group（n=28） and model group（n=28）. The lower lobe of right lung were taken and the expressions of Beclin-1 and LC3-Ⅱ mRNA in lung tissues of rats were measured by real-time fluorescent polymerase chain reaction（PCR） at 6 h, 12 h, 24 h, 48 h and 5 d after trauma. Results Compared with the sham group, the expressions of LC3-Ⅱ and Beclin-1 mRNA in model group increased significantly at different time points. Beclin-1 and LC3-Ⅱ of model group increased significantly than sham group at 48 hours after operation [（7.532±0.03） vs（1.063±0.10）,（10.843±0.03） vs（1.091±0.11）, P〈0. 01].Conclusion Autophagy may be involved in multiple trauma of acute lung injury, like femoral shaft fracture combined with brain injury.