百草枯对人肝HepG2细胞生长和周期的影响

Effects of Paraquat on Growth and Cycle in Human Liver HepG2 Cells

为全面理解百草枯(PQ)对人肝的毒性效应,寻找新的方法增强肝对PQ的解毒能力,本文采用显微观察和流式细胞术分析了PQ处理24h对人肝HepG2细胞生长和周期的影响.结果显示,7.21mg/L的PQ处理即可抑制部分HepG2细胞的生长,23.36mg/L的PQ可导致HepG2细胞周期停滞,S期细胞明显减少、G1和G2期细胞明显增多,且细胞生长抑制和周期受阻程度与PQ处理浓度呈正相关.该结果表明,PQ对HepG2细胞的生长和周期具有明显阻滞作用,生长因子类或促增殖类药物可能有助于增强人肝对PQ的解毒能力.

In order to understand comprehensively toxic effect of paraquat（PQ）on human liver and seek a novel therapy for strengthening the detoxification capacity of liver on PQ,this paper analyzed the changes of growth and cycle in human liver HepG2 cells after 24h-PQ treatment by microscopic observation and flow cytometry.The experimental results showed that the growth of partial HepG2 cells was inhibited after the treatment of 7.21mg/L PQ.The cycle of HepG2 cells is blocked,the cells in S phrase reduced,while the cells in G1 or G2phrase increased with the treatment of 23.36mg/L PQ.And also,there was a positive correlation between the dose of PQ and the inhibition of cell growth and cycle.This results indicated that PQ has evident retardation on the growth and cycle of human liver HepG2 cells,and the drugs of growth or proliferation promoting factor were likely to strengthen the detoxification capacity of liver on PQ.