超高效液相色谱-三重四极杆质谱法用于中成药中胶类成分的检测研究

LC-MS / MS Analysis of Gelatine Ingredients in Chinese Patent Medicines

目的建立含中成药中胶类药材的检测方法。方法采用胰蛋白酶对中成药样品酶解处理,利用超高效液相-三重四极杆质谱(RRLC-QQQ)对处方中阿胶、黄明胶、龟甲胶、鹿角胶的专属性特征离子进行检测。结果方法的专属性、精密度和灵敏度均符合分析检测的技术要求。部分样品检测到了处方中规定的胶类药材成分,有的样品未检测到处方规定的胶类药材成分,而检出了处方中不应有的其他胶类成分。结论所建立的方法经方法学验证,专属性强,为含胶类药材的中成药的质量控制及标准研究提供了参考。

OBJECTIVE To establish an analysis method for identification of gelatine ingredients in Chinese patent medicines. METHODS Trypsin was used for hydrolysis of gelatins. The characteristic getalins analyses were identified by rapid resolution liquid chromatography（ RRLC） coupled to triple quadruple mass spectrometry（ QQQ-MS）. Trypsin was used for hydrolysis of gelatins. RESULTS The established present method was specific,precise and reliable. Gelatine ingredients as prescribed wereas detected for several samples. However,instead of gelatin ingredients as prescribed,unprescribed gelatine ingredients were as detected for some samples. CONCLUSION The methodology validation is performed and the results are satisfied. The method can be used for identification of donkey-hide gelatin,oxhide gelatin,deer-horn glue and tortoise-shell glue in Chinese patent medicines,and provides a scientific reference for the study of quality control method of gelatin ingredients in Chinese Patent Medicines.