PLIN基因多态性与开放式生活方式干预相互作用对成人减肥效果的影响

Effect of Interaction between PLIN Gene Polymorphisms and Open Lifestyle Intervention on Weight-loss in Chinese Han Adults

目的探讨PLIN基因多态性与开放式生活方式相互作用对汉族超重及肥胖成人减肥效果的影响。方法 109名汉族成年超重或肥胖者通过随机数字表法分为研究组(n=56)和对照组(n=53),对研究组进行22周开放式生活方式干预。所有受试者干预前和干预后分别进行体格测量和血生化指标检测,通过PCR扩增及一代测序法测定PLIN1、PLIN4、PLIN6位点的基因型。结果在所有受试者中,PLIN1以罕见基因型C为主(0.619),PLIN4以常见基因型G为主(0.606),PLIN6以常见基因型A为主(0.564),其中PLIN1与PLIN4、PLIN4与PLIN6紧密连锁(D’>0.9)。干预后,研究组女性体重指数、腰围、体脂肪率显著降低且低于对照组(P<0.05),男性仅腰围下降(P<0.05)。研究组PLIN6罕见型纯合子携带者减重/减脂量差于常见型携带者,对照组PLIN1罕见型携带者体重/体脂比常见型纯合子携带者显著增加(P<0.05);研究组女性PLIN1、PLIN4、PLIN6任一位点罕见型纯合子携带者减重/减脂量均差于常见型携带者,同时携带PLIN1/PLIN4罕见型纯合子单倍型的女性减重/减脂量差于其他单倍型,同时携带PLIN4/PLIN6罕见型纯合子单倍型的女性减脂量也差于其他单倍型(P<0.05)。结论开放式生活方式干预与PLIN基因多态性相互作用,直接影响中国汉族成年超重和肥胖者的减肥效果。

Objective To explore the effect of the interaction between PLIN gene polymorphisms andopen lifestyle intervention on weight-loss in Chinese Han adults. Methods Totally 109 overweight or obese subjects were assigned by random number table to the intervention group（ n = 56） or control group（ n = 53）,and subjects in the intervention group received 22-week open lifestyle intervention. Anthropometric and metabolic indicators were measured for all subjects before and after intervention,and the PLIN1,PLIN4,and PLIN6 genotypes were amplified by polymerase chain reaction and sequenced through the first-generation sequencing technologies. Results Among all these subjects,the rare allele C was dominant at PLIN1（ 0. 619）,the common allele G was dominant at PLIN4（ 0. 606）,and the common allele A was dominant at PLIN6（ 0. 564）,in which PLIN1 and PLIN4 as well as PLIN4 and PLIN6 were in strong linkage disequilibrium（ D＇ 0. 9）. After intervention,the body mass index,waist circumference,and body fat percent of female subjects were significantly decreased in intervention group and were lower than in control group; in male subjects,however,only the waist circumference showed significant difference with the control group（ P〈0. 05）. Subjects carrying rare allele homozygote of PLIN6 got less weight / fat loss than those carrying common alleles in intervention group,while subjects carrying rare allele of PLIN1 had more weight / fat increase than those with common allele homozygote in control group（ P〈0. 05）. Females in intervention group carrying any one of rare allele homozygotes of PLIN1,PLIN4 and PLIN6 got less weight / fat loss than those with common alleles,and female subjects carrying the rare allele homozygote haplotype of PLIN1 / PLIN4 or PLIN4 / PLIN6 got less weight / fat loss than those with other haplotypes（ P〈0. 05）. Conclusion The interaction between open lifestyle intervention and PLIN gene polymorphisms can directly influence weight-loss in Chinese Han overweight and obese adults.