信号转导与转录激活因子3表达水平与垂体腺瘤各亚型的相关性

Correlation between Expression of Signal Transducer and Activator of Transcription 3 and Pituitary Adenoma Subtypes

目的探讨信号转导与转录激活因子3(STAT3)表达水平和垂体腺瘤各亚型之间的相关性。方法应用实时定量PCR、Western blot以及免疫组织化学染色检测STAT3在垂体腺瘤各亚型的表达。结果 STAT3在垂体腺瘤各亚型均有表达,与其他类型肿瘤,包括催乳素、促卵泡激素/促黄体生成激素以及促肾上腺皮质激素腺瘤相比,STAT3在生长激素腺瘤表达最高,促卵泡激素/促黄体生成激素腺瘤表达最低。结论 STAT3在垂体腺瘤各亚型的差异表达表明STAT3调控的细胞特异性本质,这种特异性的调控机制值得进一步研究。

Objective To investigate the correlation between signal transducer and activator of transcription 3（ STAT3） expression and pituitary adenoma subtypes. Method The STAT3 expression profiles in different pituitary adenomas from 74 patients were determined using quantificational real-time polymerase chain reaction,Western blot,and immunohistochemistry. Results Expression of STAT3 was observed in all pituitary adenoma subtypes. The STAT3 expression level was highest in growth hormone adenoma when compared with other tumors including prolactin,follicle-stimulating hormone / luteinizing hormone-secreting adenoma,and adrenocorticotrophic hormone-secreting adenoma. The follicle-stimulating hormone / luteinizing hormone adenomas exhibited the lowest STAT3 expression levels. Conclusion STAT3 is differentially expressed in pituitary adenoma subtypes,suggesting the cell-specific features of STAT3 regulation,although further investigations are still warranted to clarify the underlying mechanisms.