基于窄带AOTF人体胃壁细胞组织的超光谱显微成像

Hyperspectral Imaging of Human Gastric Parietal Cells Based on Narrowband AOTF

TeO_2作为声光转换材料的非线性声光可调滤波器(AOTF)是一种新型分光元件,具有体积小、质量轻、通光孔径和入射孔径大、在宽范围内快速协调、衍射效率高等特点。采用TeO_2作为声光转换材料的AOTF进行超光谱成像实验。实验中衍射光中心波长和超声波频率之间的转换计算与理论推导结果非常接近。通过改变加载在AOTF的超声波频率来改变光中心波长,结合倒置光学显微镜,设计在不同的光中心波长下呈现人体胃壁组织切片的超光谱显微成像系统。实验结果表明光谱图的成像质量很高。通过比较不同超声波频率和光中心波长下人体胃壁组织切片的超光谱图像,可观察到胃壁组织细胞一些更为详细的差异。该研究提供了一种不同光中心波长下对胃壁组织细胞进行超光谱显微成像的方法,这种方法易操作性强,结果多,易于比较。

The nonlinear acousto-optic tunable filter（AOTF） based on TeO2 is a new device which can convert the ultrasonic and optical wave. It has small volume, low weight, large aperture and incident aperture, rapid coordination in a wide spectral range, and good diffraction efficiency. The AOTF based on TeO2 is used for hyperspectral imaging experiments. The conversion between diffraction of light central wavelength and ultrasonic frequency in the experiment is very close to the theoretical result. A hyperspectral microscopic imaging system, combined with an inverted optical microscope, which can exhibit histologic section of human parietal cells under different light central wavelength and different ultrasonic frequency on AOTF is designed. The experimental results show that the image quality is very good. By comparing images under different ultrasonic wave frequency and light centeral wavelength,more details and difference of cells can be found. A method to acquire hyperspectral images of cells under different light central wavelength is provided, the method is easy to operate and the results are easy to analyze.