甘肃地区新生仔猪腹泻细菌性病原的分离鉴定及其耐药性分析

Isolation,Identification and Drug Resistance Analysis of Bacterial Etiology in New Neonatal Piglets Diarrhea in Gansu Area

为了解甘肃地区导致新生仔猪腹泻的主要细菌性病原。采集甘肃省部分地区新生仔猪腹泻病料104份,采用16SrDNA扩增技术对细菌性病原进行鉴定,经标准诊断血清确定其血清型,并用药敏纸片法对细菌性病原进行耐药性分析。结果显示:在35份病料中分离出致病性大肠埃希菌,4份病料中分离得到与致病性大肠埃希菌混合感染的沙门菌;肠道产毒性大肠埃希菌的血清型主要以O78:K80(B)为主;该地区的致病性大肠埃希菌对多种抗生素产生耐药性。表明:甘肃地区新生仔猪腹泻的细菌性病原主要是大肠埃希菌,且多重耐药性严重。

To probe the main bacterial causes of neonatal piglets diarrhea, the 104 samples of feces were collected from some areas of Gansu province appearing diarrhea symptoms. The amplification technology of 16S rDNA was used to identify bacterial etiology. The serotype of enterotoxigenic E. colic was confirmed by standard diagnostic serum and drug resistance analysis of bacterial etiology was performed using disk diffusion test. The results showed that the pathogenic E. colic was isolated in 35 samples,Salmanella and E. colic were isolated at the same time in 4 samples;The advantage serotype of enterotoxigenic E. colic was 078 ：K80 （B） and the pathogenic E. colic of Gansu area had appeared high resistance. The results suggested that the main bacterial etiology was pathogenic E. colic and multidrug resistance were more serious in Gansu area.