罗布麻查尔酮合成酶基因克隆及序列分析

Cloning and Sequence Analysis of Chalcone Synthase Gene(CHS)from Apocynum venetum

为了解罗布麻查尔酮合成酶基因具体结构，采用RT-PCR、RACE方法从夹竹桃科植物罗布麻中扩增出CHS基因的开放阅读框，其核苷酸序列长1170bp，推测的氨基酸序列全长为389个氨基酸残基。核苷酸序列同源性分析结果显示，该cDNA片段与其他植物CHS基因的同源性为78％～81％，表明该基因在进化过程中变异程度较小，整个超基因家族序列高度保守。

The main purpose of this study was to determine the structure of CHS in Apocynum veneturn. Through RT-PCR（reverse transcription polymerase chain reaction） and RACE（rapid amplification of cDNA ends）, a fragment of 1 170 bp,coding 389 amino acids（aa）, was obtained from Apocynum venetum. The nucleotide sequence of AvCHS cDNA shared 78%-81% identity with that in the other family members, which suggested that the AvCHS genes has lesser variation during evolution, and highly conserved sequence existed in the supergene family.