梨小食心虫actin基因全长cDNA克隆与序列分析

cDNA Cloning and Sequence Analysis of the Actin Gene from Grapholita molesta(Busck)

为梨小食心虫其他基因表达调控研究提供内参基因以及actin基因的研究，运用RT—PCR和RACE技术，克隆获得梨小食心虫actin基因全长cDNA序列，并对其进行生物信息学分析。结果表明，该基因cDNA序列全长为1451bp，其中包括67bp的5q}编码区、253bp的3＇非编码区和1131bp的开放性阅读框，编码376个氨基酸。该蛋白预测分子质量为41．7768ku，等电点为5．22，氨基酸序列中有6类功能位点，具有actin家族典型特征，与其他昆虫actin氨基酸序列高度同源，达97％～99％。成功克隆梨小食心虫actin基因全长cDNA，该序列已提交GenBank，登录号为KF022227。

The full-length cDNA encoding an actin was studied in order to research actin gene and provide an internal standard for other gene in Grapholita molesta. The gene was isolated by using reverse transcription-polymerase chain reaction（RT-PCR） and rapid amplification of cDNA ends-PCR（RACE- PCR）. The results of sequence analysis indicated that the cDNA was 1 451 base pairs（bp） long,con- taining 5r-untranslated region,（5＇-UTR） of 67 bp and 3＇-untranslated region, （3＇-UTR） of 253 bp. The open reading frame（ORF） of Gmolactin was 1 131 bp,encoding 376 amino acid residues,with the deduced MW of 41. 776 8 ku and PI of 5.22. The deduced protein contains 6 functional sites,shares the typical actin family signature. The deduced amino acid sequence showed a high identity to other insect（97%-99%）. The Gmolactin was successfully cloned and has been submitted to GenBank（Accession number： KF022227）.