苦瓜甾醇对对乙酰氨基酚致小鼠肝损伤的保护作用

Protective Effect of Phytosterol from Momordica charantia on Paracetamol-induced Hepatic Damage in Mice

本文研究苦瓜甾醇对对乙酰氨基酚所致小鼠肝损伤的保护作用及其作用机制。48只小鼠随机分为6组(n=8):正常对照组、模型组、阳性对照组和苦瓜甾醇低、中、高剂量(50、100和200 mg/kg)组。各剂量组小鼠给予苦瓜甾醇连续灌胃7 d后,一次性腹腔注射对乙酰氨基酚400 mg/kg制备肝损伤模型,测定肝组织和血清的生化指标。与各苦瓜甾醇组比较,模型组小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)水平显著升高,白蛋白(ALB)、总蛋白(TP)含量显著降低,肝组织中丙二醛(MDA)含量升高,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性下降。苦瓜甾醇有助于维持小鼠体内酶防御系统功能,提高清除自由基的能力,对对乙酰氨基酚所致小鼠肝损伤具有明显保护作用。

The aim of this study was to investigate the protective effect of phytosterol from Momordlca charantia on paracetamol-induced hepatic damage in mice. Forty-eight mice were randomly divided into normal control group, model group, bifendate group, phytosterol low, medium and high-dose （50,100, and 200 mg/kg） groups, with eight mice in each group. Mice in phytosterol low,medium and high-dose groups were administered with phytosterol for 7 d consecutively. After 7 days, all the mice were then intrapeHtoneally given paracetamol at a dose of 400 mg/kg. The levels of MDA, SOD, GSH-PX in hepatic tissue and ALT, AST,ALB,TP in serum were measured. Compared with phytosterol groups, in the model group, the level of MDA increased while activities of SOD and GSH-PX decreased in hepatic tissue ; the activities of ALT, AST in serum increased significantly while the levels of ALB, TP decreased. Phytosterol had good protective effect on paraeetamol-induced hepatic damage in mice. Enhancing defense enzyme activities and eliminating free radical in mice may be the action mechanism of phytosterol.