组培霍山石斛、野生霍山石斛及河南石斛多糖及乙醇溶出物动态积累规律研究

Accumulation of Polysaccharides and Ethanol-soluble Extract in Tissue-cultured and Wild Dendrobium huoshanense and Dendrobium henanense

为了揭示不同种源的霍山石斛及其近缘种河南石斛多糖和醇溶性浸出物含量的动态变化规律,为霍山石斛和河南石斛规范化种植及品种鉴定提供依据,采用蒽酮-浓硫酸比色法和醇溶性浸出物热浸法对1~4年株龄的野生霍山石斛、组培霍山石斛和河南石斛的多糖和浸出物含量进行测定。结果表明,不同株龄的组培霍山石斛、野生霍山石斛和河南石斛的多糖、浸出物含量均存在极显著差异。以多糖含量为标准,三者均以生长2年采收为宜,以醇溶物为标准,以栽培三年采收最好。以多糖和醇溶出物总量计,组培霍山石斛以2年采收为宜,野生霍山石斛及河南石斛以3年株龄品质最佳。

In order to reveal the accumulation of pharmaceutical constituents in different origins of Dendrobium huoshanense and its main counterfeit herb Dendrobium henanense, and to provide references for their standardized planting and cuhivar identification, the contents of polysaceharides and ethanol-soluble extract in different ages of tissue-cultured and wild D. huoshanense as well as D. henanense were detected by anthrone-sulfuric acid eolorimetric and hot-maceration method,respeetively. The results showed that the accumulation of polysaccharides and ethanol-soluble extract in the 12 Dendrobium samples varied remarkably as their ages increased. It was better to harvest when they were cultivated for two years according to their polysaccharide contents. It was better to harvest when they were cultivated for three years accord- ing to their ethanol-soluble extract contents. It was more reasonable to harvest tissue-cultured D. huoshanense cultivated for two years while it was better to harvest wild D. huoshanense and D. henanense cultivated for 3 years according the total contents of polysaccharides and ethanol-soluble extract.