摘要
目的观察Neuregulin-1(NRG-1)对正常小鼠心室肌细胞钙瞬变的影响。方法 9只雄性C57BL/6小鼠,随机分为3组:空白组、NRG-1组、异丙肾上腺素组(即ISO组)。分别取其心脏,经主动脉逆行插管后行Langendorff灌流,以常规酶解法分离获得单个心室肌细胞,负载Fluo-4钙荧光染料后,分别先后给予各组细胞0.5、1 Hz的电场刺激,运用活细胞工作站,记录每组细胞荧光强度的变化。结果与空白组相比,NRG-1组能显著升高钙瞬变幅值ΔF/F0(n=10,P<0.05),能缩短钙恢复时间RT50(从刺激开始至恢复到基线50%的时间)(n=10,P<0.05);与ISO组相比,NRG-1组钙瞬变幅值、钙恢复时间、达峰时间均无显著差异(n=10,P>0.05)。结论 NRG-1能升高正常心肌细胞钙瞬变幅度,缩短钙瞬变时程。
Objective To observe the effects of neuregulin-1（NRG-1）on calcium transients in mouse ventricular myocytes.Methods Nine C57BL/6mice of male were randomly dived into 3groups：the blank control group,NRG-1group,and isoproterenol（ISO）group.Their hearts were removed and immediately cannulated via the aorta and retrogradely perfused with enzymatic isolation solution to get single ventricular cell by Langendorff system.Myocytes were loaded with the Ca2＋indicator Fluo-4and subjected to electrical field stimulation at 0.5or 1Hz by using living cells workstation.The change of fluorescene intensity was recorded simultaneously in each group.Results Compared with the blank control group,NRG-1group had significantly increased Ca2＋transient amplitudeΔF/F0（n=10,P〈0.05）,and reduced Ca2＋transient recovery time RT50（n=10,P0.05）.There were no significant differences in Ca2＋transient amplitude,Ca2＋transient recovery time and the time to peak between NRG-1and ISO groups（n=10,P〉0.05）.Conclusion NRG-1can increase Ca2＋transient amplitude and Ca2＋transients and reduce the time interval of Ca2＋transients.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2015年第6期652-655,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
湖北省自然科学基金资助项目(No.2013CKB005
2014CKB497)
