摘要
目的探讨miRNA-206在胃间质瘤中的表达及意义。方法收集胃间质瘤治疗后的切除标本,采用qRT-PCR方法检测53例胃间质瘤及对应正常胃组织中miRNA-206的表达,并分析其与临床病理因素的关系。结果胃间质瘤组织中miRNA-206表达显著低于对应正常胃组织(P<0.05),且核分裂数≥5/50 HPF病例的miRNA-206表达显著低于核分裂数<5/50 HPF病例的miRNA-206表达(P<0.05),Fletcher分级中/高危病例的miRNA-206表达显著低于极低/低危分级病例的miRNA-206表达(P<0.05)。结论 miRNA-206在胃间质瘤组织中表达下调,且与核分裂像及Fletcher分级密切相关,提示其在胃间质瘤的发生发展过程中发挥重要作用。
Objective To evaluate the expression and significance of miRNA-206 in the development of gastric stromal tumor. Methods Fifty-three cases of the surgical specimens of gastric stromal tumor were collected. miRNA-206 levels in matched pairs of gastric stromal tumor and normal adjacent tissue samples were examined using qRT-PCR; the relationship between miRNA-206 levels and clinical characteristics were investigated. Results Expression of miRNA-206 was significantly lower in tumor than that in adjacent normal tissues（ P〈0. 05）. Expression of miRNA-206 in cases with mitotic count≥5 /50 HPF was significantly lower than that with mitotic count 5 /50HPF（ P〈0. 05）. By Fletcher risk stratification system,expression of miRNA-206 in intermediate / high-risk cases was significantly lower than that in very low/ lowrisk cases（ P〈0. 05）. Conclusion In gastric stromal tumor,miRNA-206 expression is significantly down-regulated,and is closely associated with mitotic counts and Fletcher risk stratification system,which suggests that miRNA-206 play an important role in the process of development of gastric stromal tumor.
出处
《山东大学学报（医学版）》
CAS
北大核心
2015年第12期67-70,共4页
Journal of Shandong University:Health Sciences
基金
国家自然科学基金(81472685)
