O-糖基化在口腔鳞状细胞癌发生发展中的作用初探

Research on the relationship between O-Glc NAc and oral squamous cell carcinoma

目的:通过口腔鳞癌组织和细胞学的研究,初步探讨O-糖基化和口腔鳞状细胞癌关系。方法:对30例口腔鳞状细胞癌和10例正常口腔黏膜组织,采用免疫组织化学SP法检测O位N-乙酰葡萄糖胺(O-linked N-acetylglucosamine,O-GlcNAc)、N-乙酰氨基葡萄糖转移酶(O-Glc NAc transferase,OGT)和N-乙酰氨基葡萄糖苷酶(O-Glc NAcase,OGA)的表达,用免疫荧光、Western blotting检测在糖基化增强剂(transglycosylation,TG)和糖基化抑制剂(deoxynorleucine,DON)作用下舌鳞状细胞癌TCA8113细胞的增殖。结果:O-Glc NAc和OGT在正常口腔黏膜组织和口腔鳞癌组织中存在表达显著性差异(P<0.05),并且随着肿瘤恶性程度的增高,呈现表达升高的趋势。OGA的表达和O-Glc NAc、OGT均不同,从正常到鳞癌中表达呈增高趋势,但在不同分化鳞癌组内表达差异不明显。TG可促进TCA8113的增殖和O-Glc NAc、OGT的表达,DON可抑制TCA8113的增殖和O-Glc NAc、OGT的表达。结论:O-糖基化在口腔鳞状细胞癌中表达增高,在舌鳞癌细胞系中活化,抑制剂DON可抑制肿瘤细胞增殖。

Objective： To investigate the relationship between O-linked N-acetylglucosamine（ O-Glc NAc） and oral squamous cell carcinoma. Methods： The expression of O-Glc NAc,O-Glc NAc transferase（ OGT） and O-Glc NAcase（ OGA） in 30 cases of oral squamous cell carcinoma and 10 normal oral mucosa were detected using immunohistochemical staining. The proliferation of TCA8113 with transglycosylation（ TG） and deoxynorleucine（ DON） was examined by Immunofluence and Western blotting. Results： There was significant difference of O-Glc NAc and OGT between normal mucosa and oral squamous cell carcinoma（ P〈0. 05）. The expression of OGlc NAc and OGT increased with higher grade of the carcinoma. The expression of OGA was inconsistent with O-Glc NAc and OGT. TG could activate the expression of O-Glc NAc and OGT,DON could inhibit the expression of O-Glc NAc and OGT. Besides,DON could inhibit the proliferation of TCA8113 cells and the expression of PCNA. Conclusions： O-Glc NAc could activate the oral squamous cell carcinoma. Inhibitor DON could depress the proliferation of TCA8113.