外源性拮抗神经纤毛蛋白1对人舌癌细胞SCC9细胞迁移及分化的影响

Effects of exogenous inhibition of Neuropilin-1 on the migration and the differentiation of human tongue squamous cell carcinoma cell line SCC9

目的:利用小分子肽ATWLPPR(A7R)对人舌癌细胞SCC9进行靶向拮抗其神经纤毛蛋白1(Neuropilin-1,NRP-1),研究其对人舌癌细胞SCC9迁移、分化的影响。方法:通过免疫印迹实验检测NRP-1蛋白在舌癌组织及人舌癌细胞SCC9中的表达情况,利用划痕实验及Transwell检测小分子肽A7R对SCC9细胞迁移的影响,以及利用实时定量荧光PCR检测相关上皮、间充质标记蛋白的m RNA表达变化,研究小分子肽A7R对SCC9细胞分化的影响。结果:NRP-1在SCC9中呈高表达,A7R拮抗后SCC9细胞迁移能力明显下降,同时SCC9细胞上皮相关标记蛋白(E-cad,β-cate)m RNA表达水平增高,而间充质相关标记蛋白(snail,FN,α-SMA)m RNA表达水平下降。结论:外源性拮抗SCC9细胞NRP-1可抑制其迁移能力并影响其细胞分化。

Objective： To investigate the effect of exogenous inhibition of Neuropilin-1（ NRP-1） on the migration and the differentiation of the human tongue squamous carcinoma cell line SCC9. Methods： Western blot was performed to examine the expression of NRP-1 in cell line SCC9 and the human tongue squamous carcinoma cell cancer tissue. Besides,scratch test and transwell assay were employed to show the influence of exogenous inhibition of NRP-1 on the migration of the SCC9 cells. And Real-time RT PCR was used to detect the expression of differentiation markers in the SCC9 cells exogenously inhibited of NRP-1. Results： NRP-1 expressed at high level in the SCC9 cells,and exogenous inhibition of NRP-1 depressed the migration of SCC9 cells. Real-time RT PCR showed the expression of epithelial cadherin and β-catenin increased,while the expression of snail,fibronectin and α-smooth muscle actin decreased.Conclusions： Exogenous inhibition of NRP-1 depressed the migration and differentiation of SCC9 cells.