茜草精油抗氧化及DNA损伤保护效果

Antioxidant Activity and DNA Damage Protective Effect of Essential Oil from Rubia cordifolia

以水蒸气蒸馏法提取茜草精油,以天然和合成的抗氧化剂为对照,用5种不同的抗氧化体系(DPPH、·OH和O^(2)-·自由基,还原力和总抗氧化能力)检测评价茜草精油的抗氧化活性;并以·OH自由基介导的p BR322质粒损伤模型进行了精油的DNA损伤保护效果的评价。结果表明:精油表现出在不同体系中以剂量依赖方式的不同程度的功效,并且对清除DPPH和·OH自由基活性较强。精油对·OH自由基介导的p BR322质粒损伤的保护效果可能源于精油对·OH自由基较好的清除作用。

The antioxidant activity and DNA damage protective effect of the essential oil from the roots of Rubia cordifolia（REO） by hydrodistillation were studied. Five different antioxidant systems,evaluated by DPPH radical method,·OH and O^2-· scavenging activity,reducing power assay and the FRAP assay were employed in order to evaluate the antioxidant activities of the essential oil as compared with natural and synthetic antioxidants. The DNA damage protective activity of essential oil was evaluated by p BR322 plasmid induced by ·OH radicals. The results revealed that the oil exhibited varying degrees of efficacy in each assay and a dose-dependent free-radical-scavenging activity against DPPH radical,superoxide anions and hydroxyl radical,as well as the oil exerted more potent radical scavenging activity against DPPH and ·OH radicals than that against O^2-·radical. The essential oil exhibited significant protective activity against ·OH radical induced DNA damage. The mechanism may be originated from the superior ability of essential oil to scavenge·OH radicals.