趋化素样因子超家族成员5对前列腺癌细胞EG-VEGF的影响

Effects of CMTM5 on EG-VEGF in prostate cancer cells

目的探讨趋化素样因子超家族成员5（CKLF-like MARVEL transmembrane domain containing 5，CMTM5），对前列腺癌细胞中内分泌腺来源的血管内皮生长因子（endocrine gland-derived vascular endothelial growth factor，EG．VEGF）表达的影响，从而探索CMTM5抑制前列腺癌的机制。方法分别检测前列腺细胞、前列腺癌细胞CMTM5、EG—VEGF的相对表达量，并对前列腺癌细胞进行CMTM5质粒转染，使其过表达CMTM5，并再次检测EG．VEGF的表达量，进行转染前后的对比。结果与正常前列腺细胞相比，前列腺癌细胞高表达EG-VEGF，低表达CMTM5，差异有统计学意义（P〈0．05）；与前列腺癌细胞相比，前列腺癌细胞转染CMTM5质粒后，EG—VEGF的表达量明显下降，差异有统计学意义（P〈0．05）。结论前列腺癌细胞较正常前列腺癌细胞高表达EG．VEGF，低表达CMTM5；当前列腺癌细胞转染CMTM5质粒，高表达CMTM5后，EG．VEGF的表达量明显下降。说明高表达CMTM5的前列腺癌细胞可能通过降低EG—VEGF途径，抑制前列腺癌的发生发展。

Objective To investigate the effect of CKLF-like MARVEL transmembrane domain contai- ning member 5 （ CMTM 5 ） on EG-VEGF expression in prostate cancer cells, and to detect the potential mechanism of CMTM5 inhibiting prostate cancer. Methods The relative expression of CMTM5 and EG-VEGF in prostate cells and prostate cancer ceils was detected. Prostate cancer cells were given Plasmid transfection to overexpress CMTM5 and EG-VEGF expression was again detected. Then CMTM5 and EG-VEGF were compared between be- fore and after CMTM5 plasmid transfecting prostate cancer ceils. Results Compared with the relative expresion of EG-VEGF and CMTM5 in prostate cells, prostate cancer cells showed high expression of EG-VEGF and low ex- pression of CMTM5, which were statistically significant, P 〈 0. 05. Compared with prostate cancer cells, the rela- tive expression of CMTM5 were obviously upregulated and EG-VEGF obviously decreased in prostate cancer cell after transfected by CMTM5 plasmid, which were statistically significant, P 〈 0.05. Conclusions Prostate canc- er cells shows higher EG-VEGF expression and lower CMTM5 campared with normal prostate cells. EG-VEGF is suppressed significantly when the prostate cancer cell is transfected by CMTM5 plasmid and shows highlevel of CMTM5 expression, suggesting high expression of CMTM5 may inhibit the development of prostate cancer by downregulating EG-VEGF expression.