茶蜂花粉提取物BPE对LPS诱导的Raw 264.7细胞的体外抗炎症作用

In Vitro Anti-Flammatory Effects of Camellia Sinensis Bee Pollen Extract(BPE) on LPS-Induced Inflammation in RAW 264.7 Macrophage Cells

以茶蜂花粉提取物(BPE)为实验材料,研究了其可能的抗炎活性。通过福林酚法和三氯化铝比色法测定BPE中的总酚酸和总黄酮质量分数,ABTS自由基清除实验评价BPE体外自由基清除能力,并采用LPS诱导的小鼠Raw 264.7细胞体外炎症模型,探究BPE对炎症因子和相关基因表达的调控作用。结果显示,BPE的总酚酸及总黄酮质量分数分别为(255.23±21.43)mg/g(绿原酸当量)和(132.85±14.77)mg/g(芦丁当量);BPE的ABTS自由基清除力的IC_(50)值为(53.9±5.38)μg/m L;在体外抗炎实验中,BPE能显著抑制Raw 264.7细胞NO的释放,显著抑制i NOS、IL-1β和IL-10,增强HO-1基因mRNA水平的表达,并呈一定的剂量相关性。表明,BPE富含黄酮和酚酸类物质并具有很强的自由基清除能力,在体外细胞实验中表现出了良好的抗炎症效果。

This study intended to evaluate the anti-inflammatory activity of Camellia sinensis bee pollen extract（BPE）. Total phenolic content and total flavonoid content of BPE were determined by the Folin-Ciocalteu method and AlCl_3 colorimetry,respectively. Meanwhile, ABTS free-radical scavenging methods were used to evaluate its free-radical scavenging capacity. On the other hand,the anti-inflammatory effect of BPE was investigated in vitro by evaluating its modulating effects on the key inflammatory cytokines and the m RNA expression of inflammatory-mediators and cytokine genes in LPS stimulated Raw 264.7 cells. The results showed that the total phenolic content and total flavonoid content of BPE were 255.23 ±21.43 mg/g and 132.85 ±14.77 mg/g,respectively. BPE exhibited strong ABTS free-radical scavenging capacity and its IC_（50） value was 53.9 ±5.38 μg/m L.In vitro,BPE exhibited significantly to inhibit NO release and the mRNA expression of i NOS,IL-1β and IL-10,and to increase the m RNA expression of HO-1. The results indicated BPE was rich in phenolic compounds and had strong free-radical scavenging capacity,and showed a good anti-inflammatory effects in vitro.