艾蒿精油的抑菌活性和稳定性

Study on Antimicrobial Activities and Stability of Essential Oil from Artemisia argyi

采用滤纸片法测定艾蒿精油的抑菌活性,平板涂布法测定其最低抑菌浓度(MIC);同时研究了艾蒿精油抑菌活性的酸碱稳定性、热稳定性、紫外稳定性,以及壳聚糖、CaCl_2、NaCl对艾蒿精油抑菌效果的影响。结果表明:艾蒿精油对3种受试菌的抑制效果有较大差异,对革兰氏阳性菌的抑制作用较强,对革兰氏阴性菌的抑制作用较弱;精油对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌的MIC分别为2.5、5、10μL/m L。艾蒿精油具有良好的紫外稳定性;温度和pH对艾蒿精油的抑菌效果影响较大,温度低于40℃时抑菌活性较好,高于40℃,则抑菌活性逐渐下降;酸性条件下可增强艾蒿精油的抑菌活性。壳聚糖、CaCl_2、NaCl均可增加艾蒿精油的抑菌效果,且壳聚糖、CaCl_2对艾蒿精油抑菌效果的影响大于NaCl。

The filter Paper disk disffusion and spread-plate assay were used to determine the antimicrobial activity and minimum inhibitory concentration（MIC） of essential oil from Artemisia argyi. The effect of antimicrobial activity on stability of acid,base,heat and ultraviolet ray were studied. Results showed that the antimicrobial activitity of the essential oil from Artemisia argyi against three tested microbes were largely different. The essential oil showed strong antimicrobial effect against the Gram positive bacteria while weak effect against Gram negative bacteria. The MICs of the essential oil against Staphylococcus aureus,Bacillus subtilis,Escherichia coli were 2.5,5,10μL/m L,respectively. The antimicrobial activities of the essential oil were stable under the ultraviolet ray,but were unstable under different temperatures and pH values. The acitivity was much better when the temperure below 40 ℃,and strong in acid and weak in base solution. Chitosan,CaCl_2,NaCl can increase the antimicrobial activity,while Chitosan and CaCl_2 showed higher effect as compared to NaCl.