ATDC5:一株反映软骨形成完整过程的细胞系

ATDC5: A well-characterized cell line of reflecting a complete chondrogenesis progress

中医学认为,筋骨失养,系肝肾虚衰所致。补肾中药的某些有效成分具有与细胞因子、激素类似的生理活性和广泛的药理作用,不仅可用于骨性关节炎的治疗,而且在组织工程及干细胞工程领域有广泛的应用前景。ATDC5细胞株来源于小鼠畸胎癌株AT805,作为一个前软骨细胞株其分化过程与软骨形成过程类似。在细胞因子、激素和无机磷酸盐等作用下,ATDC5细胞将发生增殖、聚集进而进入软骨细胞分化阶段,分化为增殖性软骨细胞。增殖性软骨细胞随后继续分化为肥大性软骨细胞,从而进入终末分化阶段,软骨基质发生矿化,最终沉积成骨。通过从系统调节因子、局部调节因子和细胞培养条件三个方面,揭示ATDC5细胞增殖、分化与矿化的分子机制,为软骨发育研究及中药有效成分高通量筛选提供理论依据。

The malnutrition of bone and muscle is caused by the failure of liver and kidney in traditional Chinese medicine. Some effective components, isolated from nourishing kidney drugs have the widely pharmacological effects, which is similar to cytokines or hormones that are used for osteoarthritis treatment, tissue engineering and stem cell engineering. ATDC5 cell, derived from mouse teratocarcinoma AT805, is characterized as a chondrogenic cell line which goes through a sequential process analogy to chondrogenesis. Under the effects of the differentiation factors, such as cytokines, hormones, inorganic phosphate, etc, ATDC5 cells proliferate, gather and differentiate into proliferating chondrocytes. And then mineralized matrix is produced and proliferating chondrocytes subsequently differentiate into hypertrophic chondrocytes and are gradually replaced by bone. From system regulation factors,local regulation factors and cell culture conditions, this review will reveal the molecular mechanism of proliferation,differentiation and mineralization of ATDC5 cell, which provides the theory basis for cartilage development researches and high-throughput screening of effective components of Chinese traditional medicine.