寻常型银屑病患者皮损中miRNA-34a及其靶基因Notch1的表达及意义

Expression and Significance of mi RNA-34a and its Target Gene Notch1 in Lesions of Patients with Psoriasis Vulgaris

目的：探讨miRNA-34a及其靶基因Notch1在寻常型银屑病患者皮损中的表达水平及意义。方法：实时荧光定量PCR法检测22例寻常型银屑病患者皮损组织及10例正常人皮肤组织中miRNA-34a的表达水平。采用免疫组化EnVision法检测51例寻常型银屑病患者（包括上述22例患者）和29例正常人（包括上述10例正常人）皮损组织中miRNA-34a可能的靶基因Notch1的表达情况。结果：miRNA-34a在寻常型银屑病患者皮损组织中的表达水平为0.162±0.186,高于正常皮肤组织中的表达水平（0.028±0.029）,银屑病组为正常对照组的5.786倍,两组比较,差异有统计学意义（t=2.22,P〈0.05）;Notch1在寻常型银屑病组织中主要为表皮全层细胞质表达,阳性表达率为76.47%（39/51）,而在正常皮肤组织中阳性表达率为13.79%（4/29）,两者差异有统计学意义（χ^2=29.22,P〈0.05）。结论：miRNA-34a在寻常型银屑病皮损组织中的表达高于正常人皮肤组织,其可能通过调控靶基因Notch1参与寻常型银屑病的发病机制。

Objective： To investigate the expression and significance of miRNA-34 a and its target gene Notch1 in lesions of patients with psoriasis vulgaris. Methods： Real-time fluorescent quantitative PCR was performed to determine the expression level of miRNA-34 a in 22 patients with psoriasis vulgaris and normal skin of 10 healthy human controls. Potential target genes of mi RNA-34 a were found using software of mi Rwalk to predict target gene,then the expression of Notch1 was detected by immunohistochemistry in 51 patients with psoriasis vulgaris（ including 22 patients above） and 29 healthy controls（ including 10 healthy people above）. Results： Real-time fluorescent quantitative PCR showed that the expression level of miRNA-34 a was higher in lesions of psoriasis vulgaris than that in the normal skin [（ 0. 162 ± 0. 186） vs（ 0. 028 ± 0. 029）,times：5. 786,t = 2. 22,P〈0. 05]. The Notch1 was expressed in epidermal cytoplasm at full-thickness of epidermis in lesions of psoriasis vulgaris and the positive rate was 76. 47%（ 39 /51）,while the positive rate was 13. 79%（ 4 /29） in normal skin. There was statistical significance between the two groups（ χ^2= 29. 22,P〈0. 05）. Conclusion： The expression level of mi RNA-34 a was significantly up-regulated in lesions of psoriasis vulgaris,which might be regulated its target genes Notch1 and played a part in the pathogenesis of psoriasis vulgaris.