聚合酶链反应在44例非典型临床表现的急性结膜炎病因诊断中的意义

Etiological diagnosis of atypical acute conjunctivitis with polymerase chain reaction

[目的]探讨聚合酶链反应(PCR)在非典型临床表现的急性结膜炎病因诊断中的意义.[方法]选择非典型急性结膜炎患者44人(44眼)及正常人22人(22眼),两眼共患结膜炎者取较重一侧.提取受试者结膜囊分泌物的DNA,分为2部分,分别加入细菌引物和腺病毒引物进行2次PCR扩增,凝胶电泳后观察结果.非典型急性结膜炎组同时进行细菌培养.[结果]非典型急性结膜炎组12眼(27%)扩增出520bp条带,11眼(25%)扩增出1 004bp条带,扩增出520bp兼1 004bp条带的有3眼(7%).细菌培养法有17眼(39%)呈阳性,与PCR检测结果比较差异无统计学意义(χ2=1.77,P>0.05).正常组1眼(5%)扩增出520 bp条带,未扩增出1 004 bp条带.[结论]PCR检测对非典型急性结膜炎快速病因诊断具有临床意义.

OBJECTIVE To discuss the clinical significance of polymerase chain reaction（PCR）for etiological diagnosis of atypical acute conjunctivitis（AAC）.METHODS44 eyes from 44 patients with AAC and 22 eyes from 22 healthy persons were chosen,in which the more severe one of two eyes were taken.All DNA were extracted from secreta of conjunctival sac in subjects and divided into two parts,and the PCR amplifications were separately carried out with bacteria primer and adenovirus primer to observe the results with gel imaging analysis.Meanwhile,bacterial culture of the patients with AAC is performed.RESULTS520 bp stripes were amplified in 12eyes（27%）in AAC group,and 1 004 bp stripes in 11eyes（25%）,and both 520 bp and 1 004 bp stripes were amplified at the same time in 3eyes（7%）,and 520 bp stripes with no1 004 bp stripes were amplified in 1eye（5%）in normal group.Positive results were shown in 17eyes（39%）with bacterial culture with no statistical significance as compared with the PCR test results.CONCLUSIONPCR can be used in the rapid etiological diagnosis of AAC.