大豆GmDGK7和GmTPR基因与油脂相关性状的关联分析

Association of GmDGK7 and GmTPR Genes with Oil Related Traits in Soybean

根据已知油脂相关同源基因分析,选择两个油脂代谢相关基因,分别编码甘油二酯激酶7(diacylglycerol kinase7,DGK7)和TPR蛋白(pentatricopeptide repeat-containing protein,TPR),并根据两个基因内部及非翻译区(untranslated region,UTR)的SNP变异位置和突变类型,选取位于GmDGK7基因5'-UTR区的一个单核苷酸变异(SNP1)及位于Gm TPR基因外显子区的SNP2设计d CAPS标记。对200份育成大豆品种的SNP1和SNP2分型及其与大豆籽粒油脂相关性状的关联分析发现,GmDGK7基因的SNP1与油脂含量显著关联(P=6.21×10-5),GmTPR基因的SNP2与油脂含量、硬脂酸、油酸和亚油酸均显著关联(P值分别为4.9×10-11,1.6×10-6,3.4×10-5,4.09×10-8)。此外还筛选出14份高油脂(>23%)和5份高油酸(>33%)大豆种质,这些分子标记和种质资源可为改良大豆油脂性状和分子育种提供技术和材料基础。

Soybean is the most important oil crop in the world,which oil production accounts for more than a quarter of global vegetable oil production,thus increasing the oil content and oil composition in soybean seed is essential for soybean breeding.However,there are limited molecular markers derived from functional genes in soybean breeding program. Two soybean genes that are homologous to known proteins involved in oil metabolic pathways were chosen to develop molecular markers in this study,including genes encoding a diacylglycerol kinase 7（ DGK7） and a pentatricopeptide repeat-containing protein（ TPR）,respectively. Based on the position and type of mutation of the single nucleotide polymorphisms（ SNPs） within these two genes,we selected SNP1 located in the 5＇- untranslated region（ 5＇-UTR） of Gm DGK7 and SNP2 located in the Gm TPR exon to design d CAPS markers. Association mapping of SNP1 and SNP2 with the oil related traits among 200 soybean released cultivars showed that SNP1 in Gm DGK7 is significantly（ P = 6. 21 × 10- 5） associated with oil content,SNP2 in Gm TPR is significantly associated with the content of oil,stearic acid,oleic acid and linoleic acid（ P values were 4. 9 × 10- 11,1. 6 × 10- 6,3. 4 × 10- 5and 4. 09 × 10- 8,respectively）. Fourteen soybean cultivars with high oil content（ 23%） and 5 cultivars with high oleic acid（ 33%） were identified in this mapping population. These cultivars and d CAPS markers could be used in marker-assisted breeding to improve soybean oil content and quality.