重组骆驼蓬脂转移蛋白与顺铂联合抑制黑色素瘤B16细胞增殖效应的研究

The Synergistic Inhibitory Effects of Recombinant Peganum Harmala Lipid Transfer Protein and Cisplatin on the Proliferation of Melanoma B16 Cells in vitro

旨在探讨重组骆驼蓬脂转移蛋白(Recombinant Peganum harmala lipid transfer protein,r Ph LTP)与顺铂(DDP)联用对鼠黑色素瘤B16细胞增殖抑制和凋亡诱导作用。MTT法检测r Ph LTP、顺铂单独及联用后对鼠黑色素瘤B16细胞增殖的影响;流式细胞术检测细胞凋亡,活性氧(Reactive oxygen species,ROS)和线粒体膜电位(Δψm)的变化情况。结果显示,r Ph LTP和DDP联用后细胞生长抑制率和凋亡率显著高于DDP单独处理组(P<0.01);而且胞内ROS升高的水平均高于单独处理组(P<0.01),胞内Δψm水平均低于单独处理组,但无显著性差异。r Ph LTP可增强顺铂对B16细胞的生长抑制并促进细胞凋亡,与顺铂具有协同作用。

This work is to explore how the combination of recombinant Peganum harmala lipid transfer protein（r Ph LTP）and cisplatin（DDP）inhibits the proliferation of melanoma B16 cells and induces the apoptosis of them. MTT assay was used to measure the effects of individual r Ph LTP and DDP and combination of them on the proliferation of melanoma B16 cells. The cell apoptosis rate, reactive oxygen species（ROS）, and mitochondrial transmembrane potential（Δψm）level were measured using flow cytometry. The results showed that both inhibitory rate and apoptosis rate by the combination of r Ph LTP and DDP were significantly higher（P 0.01）than by individual DDP or r Ph LTP, moreover also levels of ROS raised inside cells by the combination were higher than by individuals（P 0.01）. However, the levels of Δψm inside the cells by the combination were lower than those by individuals, but the differences between the combination and individuals were not such significant. In conclusion, r Ph LTP enhanced the inhibitory effect of DDP on the proliferation of B16 cells and promoted the cell apoptosis, indicating that r Ph LTP and DDP has synergistic antitumor effect on B16 cells.