不同温度培养下酿酒酵母细胞壁蛋白质差异分析

A Protein Differential Analysis of Cell Wall in Saccharomyces cerevisiae Under Different Temperatures

生物体在其生长过程中要经受一系列非生物环境的胁迫,这些胁迫条件都将影响细胞的基因转录、蛋白质表达物等一系列的变化,以尽快适应周围变化的环境。利用双向电泳和质谱技术考察了高温胁迫对酿酒酵母细胞壁蛋白质组的影响。结果表明,高温胁迫的酿酒酵母FFC2146细胞壁蛋白质中新增Ssa2和小分子鸟苷三磷酸酶,无机焦磷酸酶上调表达,而丙酮酸激酶缺消失,同时6-磷酸葡萄糖酸脱氢酶和3-磷酸甘油醛脱氢酶下调表达。上述结果说明热休克蛋白Ssa2保护细胞壁在高温下保持完整,使细胞继续生长繁殖;高温胁迫下酿酒酵母的糖酵解途径受阻,在转酮醇酶的作用下糖酵解途径转向磷酸戊糖途径途径,获取足够的能量,维持细胞正常的新陈代谢。

Living organisms undergo a series of stresses from abiotic environment, and these stresses will affect the changes of genetic transcription and the expression of protein for quick adaptation to the changing environment. Two-dimensional electrophoresis and mass spectrometry were used to investigate the effects of high-temperature stresses on the proteome of cell wall in Saccharomyces cerevisiae. The results showed that, in S. cerevisiae FFC2146 under high temperature, proteins of cell walls had new Ssa2 and small molecules guanosine triphosphatase, inorganic pyrophosphatase was up-regulated, pyruvate kinase was deficient or even disappeared, and the expression of glucose-6-phosphate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase was down-regulated. These results indicated that the heat shock protein Ssa2 protected cell walls to be intact at a high temperature, therefore the cells grew and reproduced continuously;EMP glycolytic pathway of S. cerevisiae under heat stress was blocked, glycolytic pathway turned HMP pathway by transketolase, enough energy was obtained to maintain normal metabolism of cell.