常压室温等离子体诱变选育类胡萝卜素高产菌株及其性质研究

The Screening and Characterization of High-yield-carotenoid Strain from Deinococcus wulumuqiensis R12 by ARTP

采用新型常压室温等离子体(Atmospheric and room temperature plasma,ARTP)诱变产类胡萝卜素菌株Deinococcus wulumuqiensis R12,通过深孔培养板进行高通量筛选,并结合菌株类胡萝卜素含量复筛获得突变菌株M1。与出发菌株相比,突变菌株M1的类胡萝卜素含量检测为612μg/g细胞干重(Dry cell weight,DCW),是出发菌株类胡萝卜素含量(212μg/g DCW)的2.8倍,且遗传性稳定;突变菌株M1类胡萝卜素的高铁离子还原能力(OD700=0.52)高于出发菌株类胡萝卜素的高铁离子还原能力(OD700=0.34),且M1的类胡萝卜素对DPPH自由基清除率为33.33%高于出发菌株类胡萝卜素的清除率(23.09%),结果显示突变菌株M1具有更强的抗氧化能力;在相同γ辐射与UV辐射剂量下突变菌株M1具有比出发菌株更高的存活率。研究表明突变菌株M1在类胡萝卜素产量、菌株抗氧化性能及抗辐射性能方面均优于出发菌株D.wulumuqiensis R12。

Using the atmospheric and room temperature plasma（ARTP）to induce the carotenoid-producing Deinococcus wulumuqiensisR12, the mutant strain M1 was finally obtained by high-throughput screening with polystyrene plates as well as re-screening according to the content of carotenoid. The strain M1 produced 612 μg/g dry cell weight（DCW）of carotenoid after fermenting 72 h, 2.8 times of carotenoid by original D. wulumuqiensis R12（212 μg/g DCW）, and heredity was stable. The carotenoid of the strain M1 showed stronger activity in high iron ion reducing power（OD700 = 0.52）than that（OD700 = 0.34）of original strain, and higher DPPH scavenging rate（33.33%）than 23.09%, indicating the mutant strain M1 possessed better anti-oxidative capacity. Under the same γ-ray and UV radiation dose, the strain M1 survived at higher rate than the original strain. Experimental results indicated that the strain M1 was superior to original D. wulu muqiensis R12 in the aspects of carotenoid content, anti-oxidative capacity of carotenoid and anti-radiation capacity of strain.