噻虫嗪对金银花药材指标成分含量影响的初步研究

Preliminary Study on Influence of Component Content Indexes in Honeysuckle by Thiamethoxam

目的:研究内吸性化学杀虫剂噻虫嗪对金银花药材指标成分绿原酸和木犀草苷含量的影响。方法:采用高效液相色谱法对施用不同浓度噻虫嗪药剂的金银花样品指标成分进行含量测定,采用SPSS软件对其结果进行差异统计分析。结果:施用不同浓度噻虫嗪的金银花样品与空白对照组样品对比,绿原酸含量有升高趋势,但实验组间没有无显著性差异;而施用不同浓度噻虫嗪的金银花样品与空白对照样品之间木犀草苷含量没有显著性差异。结论:在推荐剂量和高倍剂量下,噻虫嗪对金银花中绿原酸的含量有一定的影响,对木犀草苷的含量没有显著影响,且二者含量的质量分数均在《中国药典》(2010版)中规定的范围内。

This study was aimed to investigate the influence on component content indexes, which included chlorogenic acid and luteolin of honeysuckle by the absorption of chemical insecticide thiamethoxam. High-performance liquid chromatography（HPLC） method was used in the analysis of content determination of component content indexes of honeysuckle samples by different concentrations of thiamethoxam. SPSS was used in the statistical analysis. The results showed that compared to samples from the blank control group, the content of chlorogenic acid had the increasing tendency in the honeysuckle groups with different concentrations of thiamethoxam. However, there was no significant difference among different experiment groups. There was no significant difference on luteolin among honeysuckle groups with different concentrations of thiamethoxam and the blank control group. It was concluded that at the recommended dose and high-powered dose of thiamethoxam, there was some influence on the content of chlorogenic acid. However, there was no significant effect on the content of luteoloside. And the quality scores of both contents were within the range defined in the Chinese Pharmacopoeia（2010 edition）.