银杏叶有效成分抗血小板聚集和清除DPPH自由基的量效关系和协同作用

Dose-response Relation and Synergy Effects of Bioactive Components in Ginkgo biloba on Antiplatelet Aggregation and DPPH Free Radical Scavenging

目的:考察银杏叶中有效成分的抗血小板聚集、清除DPPH自由基的量效关系和协同作用。方法:分别采用抗血小板聚集实验和DPPH自由基清除实验研究量效关系和协同作用。首先,考察银杏内酯PAF诱导的家兔血小板聚集的抑制作用。然后,考察银杏黄酮对DPPH自由基清除作用。最后,考察有效成分之间的协同作用。结果:得到银杏内酯A、银杏内酯B、银杏内酯C和白果内酯抗血小板聚集的量效关系图,槲皮素、山柰酚和异鼠李素清除DPPH自由基的量效关系图和有效成分之间相互作用等效图。结论:银杏内酯抗血小板聚集具有量效关系,且银杏内酯A和银杏内酯B有协同作用;银杏黄酮清除DPPH自由基具有量效关系,且槲皮素和异鼠李素具有协同作用。

This study was aimed to investigate the dose-response relations and synergy effects of bioactive components in Ginkgo biloba on antiplatelet aggregation and DPPH free radical scavenging. The antiplatelet aggregation experiment and DPPH free radical scavenging experiment were conducted respectively to investigate the dose-response relations and synergy effects. Firstly, the effect of inhibiting platelet aggregation induced by PAF of ginkgolides in rabbits was investigated. And then, the effect of DPPH free radical scavenging by ginkgo flavonoids was also investigated. Finally, the synergy effects among effective components were studied. The results showed that ginkgolide A, ginkgolide B, ginkgolide C and bilobalide had dose-response relations on antiplatelet aggregation. Quercetin, kaempferol and isorhamnetin had DPPH free radical scavenging effects with dose-response relation. It was concluded that ginkgolide had the dose-response relation on antiplatelet aggregation. Ginkgolide A and ginkgolide B had synergy effect. Ginkgo flavonoids had DPPH free radical scavenging effect. Quercetin and isorhamnetin had synergy effect.