摘要
目的分析骨形态发生蛋白-7(BMP-7)在胃癌中的表达,初步探讨BMP-7对胃癌诊断的意义。方法以27例胃癌及相应的癌旁组织为研究对象,采用实时荧光定量PCR和Western blotting方法,分别检测组织中BMP-7 mRNA及其蛋白的表达情况。结果27例配对的胃癌及其癌旁组织中,63.0%(17/27)的癌组织BMP-7 mRNA的表达高于癌旁组织,差异有统计学意义(t=2.94,P〈0.01,配对t检验);Western blotting结果显示,77.8%(7/9)的癌组织BMP-7蛋白水平的表达高于癌旁组织,差异有统计学意义(t=2.57,P%0.05,配对t检验)。结论胃癌组织中BMP-7的表达高于癌旁组织,可能与胃癌的发生、发展相关,对临床胃癌的辅助诊断可能具有潜在价值。
Objective To preliminarily investigate the expression of BMP-7 in gastric cancer and its significance for gastric cancer diagnosis. Methods The expression level of BMP-7 mRNA in 27 pair-matched gastric neoplastic and adjacent non ne oplastic tissues was determined by quantitative reverse transcriptase polymerase chain reaction, and western blotting was performed to analyze BMP 7 protein level in randomly selected 9 pair-matched tissues. Results The relative expression level of BMP-7 mRNA in 63.0% (17/27) gastric cancer tissues was significantly higher than that in adjacent non-neoplastic tis- sues (t=2.57,P〈0.05,paired t-test). The level of BMP-7 protein in 77.8%(7/9) gastric cancer tissues was higher than that in adjacent non-neoplastic tissues (t= 2.57, P〈0.05, paired t-test). Conelasion Compared to the non neoplastic tissues,expression of BMP 7 was increased in gastric cancer,indicating BMP-7 might be associated with the occurrence and development of gastric cancer,and may have potential value for the diagnosis of gastric cancer.
出处
《现代检验医学杂志》
CAS
2015年第6期18-19,24,共3页
Journal of Modern Laboratory Medicine
基金
南京市医学科技发展重点项目(ZKX08030)、专项课题(YKK13066).
关键词
胃癌
骨形态发生蛋白-7
gastric cancer
bone morphogenetic protein-7