骨形态发生蛋白-7在胃癌组织中的表达及意义

Expression and Clinical Significance of Bone Morphogenetic Protein-7 in Gastric Cancer

目的分析骨形态发生蛋白-7（BMP-7）在胃癌中的表达，初步探讨BMP-7对胃癌诊断的意义。方法以27例胃癌及相应的癌旁组织为研究对象，采用实时荧光定量PCR和Western blotting方法，分别检测组织中BMP-7 mRNA及其蛋白的表达情况。结果27例配对的胃癌及其癌旁组织中，63．0％（17／27）的癌组织BMP-7 mRNA的表达高于癌旁组织，差异有统计学意义（t=2．94，P〈0．01，配对t检验）；Western blotting结果显示，77．8％（7／9）的癌组织BMP-7蛋白水平的表达高于癌旁组织，差异有统计学意义（t=2．57，P％0．05，配对t检验）。结论胃癌组织中BMP-7的表达高于癌旁组织，可能与胃癌的发生、发展相关，对临床胃癌的辅助诊断可能具有潜在价值。

Objective To preliminarily investigate the expression of BMP-7 in gastric cancer and its significance for gastric cancer diagnosis. Methods The expression level of BMP-7 mRNA in 27 pair-matched gastric neoplastic and adjacent non ne oplastic tissues was determined by quantitative reverse transcriptase polymerase chain reaction, and western blotting was performed to analyze BMP 7 protein level in randomly selected 9 pair-matched tissues. Results The relative expression level of BMP-7 mRNA in 63.0% （17/27） gastric cancer tissues was significantly higher than that in adjacent non-neoplastic tis- sues （t=2.57,P〈0.05,paired t-test）. The level of BMP-7 protein in 77.8%（7/9） gastric cancer tissues was higher than that in adjacent non-neoplastic tissues （t= 2.57, P〈0.05, paired t-test）. Conelasion Compared to the non neoplastic tissues,expression of BMP 7 was increased in gastric cancer,indicating BMP-7 might be associated with the occurrence and development of gastric cancer,and may have potential value for the diagnosis of gastric cancer.