摘要
目的探讨Numb基因在宫颈癌组织中的表达及对宫颈癌细胞增殖的影响。方法收集30例正常宫颈组织、56例宫颈癌组织和17例原位癌组织采用免疫组织化学染色观察Numb基因的表达情况。通过构建siRNA表达载体下调宫颈癌HeLa细胞株中内源性Numb基因表达,MTT实验检测下调Numb表达后细胞增殖能力的变化。结果免疫组织化学染色结果显示,Numb在宫颈癌及原位癌中的表达明显高于正常宫颈组织,其阳性表达率分别为72.2%,69.6%和60%,(χ^2=30.259,P〈0.05)。siRNA干扰宫颈癌HeLa细胞,干扰后Numb基因的蛋白表达水平(灰度值分别为:0.51±0.04和0.56±0.05)较对照组(灰度值分别为:0.78±0.06和0.75±0.05)明显下降(t=4.2,P〈0.05)。MTT实验显示,干扰Numb基因后HeLa细胞培养第1,3,5和7天时,增殖活力(吸光度值分别为:0.211±0.06l,0.235±0.013,0.438±0.05l和0.773±0.032)较对照组(吸光度值分别为:0.225±0.047,0.300±0.026,0.549±0.039和1.023士0.041)显著下降,差异具有统计学意义(t=5.1,P〈0.05)。结论Numb基因在宫颈癌组织中高表达,Numb在宫颈癌中可能通过促进癌细胞的增殖而促进宫颈癌的进展。
Objective To investigate the expression of Numb gene in cervical cancer and its effect on the proliferation of cervi- cal cancer cells. Methods Theexpression of Numb protein was detected in 30 normal peoples,56 cases with cervical cancer and 17 cases with carcinoma in situ by immunohistochemistry assay. The siRNA technology was used to downregulate the endogenous expression of Numb in cervical cancer HeLa cell. Then MTT assay were applied to observe the change of cell proliferation. Results The expressions of Numb in cervical cancer in situ and invasive cervical cancer were significantly higher than in normal cervix and the positive rates were 72.2% ,69.6% and 60% respectively (χ^2 =30. 259,P〈0.05). The expression of Numb was significantly decreased in HeLa cells(0.78±0.06 and 0. 75±0. 05) by siRNA (0.51±0.04 and 0.56±0.05) (t=4.2,P〈0.05). In 1, .3,5 and 7 days,MTT assay showed that cell proliferation rates were dramatically de creased in the interfered Hela cells (absorbance value:0. 211±0. 061,0. 235±0. 013,0. 438±0. 051 and 0. 773±0. 032 respectively) than the control (absorbance value:0. 225±0. 047,0. 300±0. 026,0. 549±0. 039 and 1. 023±0. 041 respective ly). Conclusion Numb was over-expressed in cervical cancer and took part in cervical cancer progress through accelerating cell proliferation.
出处
《现代检验医学杂志》
CAS
2015年第6期42-45,共4页
Journal of Modern Laboratory Medicine