大鼠X射线辐照后对血清抗氧化酶影响的实验研究

-Experimental Study on Influence on the Antioxidant Enzymes of Serum in Rates Irradiated by X-Rays

目的探讨大鼠X射线辐照后对血清抗氧化酶的影响。方法选择无特定病原体成年大鼠19只，随机分为试验组（A）、试验组（B）和对照组（C）。A，B两组大鼠均每日进行全身一次性X线照射，连续6天，1次／天。A组照射剂量为3Gy，B组照射剂量为2Gy，C组不进行X线照射，完毕后留取血样检测3组大鼠血清中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH—Px）活性，并予以统计分析。结果SOD：A组185．496士27．606U／ml，B组255．087±43．519U／ml，C组299．171±26．890U／ml，A，C组间与A，B组间差异均有统计学意义（P〈0．05），B与C组间差异无统计学意义（P〉0．05），表明A组SOD水平显著低于C组，且A组显著低于B组；CAT：A组0．438±0．400U／ml，B组1．173±0．457U／ml，C组1.497±0．702U／ml，A，C组间与A，B组间差异均有统计学意义（P〈0．05），B与C组间差异无统计学意义（P〉0．05），表明A组CAT水平显著低于C组，且A组显著低于B组；GSH—Px：A组3139．474±435．677U／ml，B组3160．150±335．989U／ml，C组3189．473±338．204U／ml，GSH-Px检测各组间差异均无统计学意义（P〉0．05），表明A和B组GSH—Px水平低于C组，且A组低于B组。结论X线辐射对血清SOD和CAT指标具有显著性影响，可显著降低血清SOD及CAT水平，抗氧化酶SOD及CAT活性与照射剂量呈负相关；X线辐射可降低血清GSH-Px水平，但对GSH-Px指标无显著性影响。

Objective To explore influence on the antioxidant enzymes of serum in rates irradiated by X-ray. Methods Ghose 19 rats without specific pathogen free and they were randomly divided into experimental group （A）, experimental group （B） and the control group （C）. A,B two group rats were daily with whole body one-time X-ray irradiation, A,B group to X-ray irradiation for 6 d consecutive time and 1 time/d, A group radiation dose were 3Oy, B group radiation dose were 2Gy, C group hadn＇t radiation. Then got blood specimens of 3 group rats and superoxide dismutase （SOD）, hydrogen peroxide enzyme （CAT） and glutathione oxidation reductase （GSH-Px） wrer detected. The data had statistics analyse. Results SOD：A group 185. 486±27. 606 U/ml,B group 255. 087±43. 519 U/ml,C group 299. 171＋-26. 890 U/ml,the difference between A and C group was statistically significant （P〈0.05） ,the difference between A and 13 group was statistically significant （P〈0.05） ,no difference between B and C groups was statistically significant （P〉0.05） ,while the level of SOD for A group was significantly below than C group, and A group was significantly below than B group. CAT： A group 0. 438± 0. 400 U/ml, B group 1. 173±0. 457 U/ml,C group 1. 497±0, 702 U/ml,the difference between A and C group was statistically significant （P〈0.05）, the difference between A and B group was statistically significant （P%0.05）, no difference between B and C groups was statistically significant （P〈0.05） ,while the level of CAT for A group was significantly below than C group,and A group was significantly below than B group. GSH-Px：A group 3 139. 474±435. 677 U/ml,B group 3 160. 150±335. 989 U/ml,C group 3 189. 473±338. 204 U/ml,there was no statistically significant difference between the groups in GSH-Px detection （P〉0. 05） ,while the level of GSH-Px for A and B group were below than C group,and A group was below than B group. Conclusion X-ray radiation had significant influence on the SOD and CAT of serum,and could significantly reduce the level of serum SOD and CAT. The antioxidant enzyme active of SOD and CAT was anticorrelation with irradiation dose. X-ray radiation can reduce the level of serum GSH-Px,there was no significant influence on the GSH-Px.