健康成人血浆D-二聚体免疫比浊法正常参考区间的建立

Establishment of the Normal Reference Range of Plasma D-Dimer Immune by Turbidimetric Method in Healthy Adults

目的建立宝安区健康成人不同年龄段血浆D-聚体免疫比浊法生物参考区间，为临床诊断和治疗提供更科学更有价值的参考依据。方法随机选取2014年6月-2015年1月深圳市宝安区福永人民医院门诊、住院及体检科不同年龄健康成人1156例，其中男性417例，女性739例。按年龄分为18-49岁，50-59岁及〉60岁三组。采用Sysmexc A1500全自动凝血分析仪免疫比浊法检测血浆D-二聚体浓度，采用SPSS19．0对数据进行统计分析，血浆D-二聚体浓度呈偏态分布，采用百分位数（P95）表示参考区间的单侧上限，确定该区相应的生物参考区间，并分析比较不同年龄和不同性别之间血浆D-二聚体浓度之间的差异性。结果同年龄组男性和女性D-二聚体浓度水平比较差异无统计学意义（t=0．372-1．092，P均〉0．05）；18-49岁组和50-59岁组健康成人血浆D-二聚体浓度水平比较差异无统计学意义（t=0．954，P〉0．05），〉60岁组与其他两组比较，血浆D-二聚体浓度差异有统计学意义（t=5．972，P〈0．05）；18-59岁组D-二聚体正常参考区间为0-225mg／L；〉60岁组D-二聚体正常参考区间为0-297mg／L。结论血浆D-二聚体浓度水平与性别无关，而与年龄有关，随年龄增高D-二聚体水平呈上升趋势。因此，不同地区应建立健康成人不同年龄段D-二聚体正常值参考区间。

Objective To establish Baoan district different age healthy adults plasma D - dimer biological reference interval, for clinical diagnosis and treatment to provide more scientific and more valuable reference. Methods From June 2014 to Jan- uary 2015,randomly selected 1 156 cases of different age,among which 417 were male,female 739 cases in the hospital out- patient and inpatient and physical healthy adults. By age,they were divided into 18 to 49,50 to 59,and 〉60 years old three groups. Used Sysmex CA1500 automatic blood coagulation analyzer immune turbidimetrie method to detect plasma D-dimer concentration,statistical analysis of the data by SPSS19.0, and concentration of plasma D-dimer was skewed distribution. Used percentile （P95） to express the reference range of unilateral cap, determine the corresponding biological reference range in this area. Then analysed and compared the differences of the concentration of plasma D-dimer in people of different ages and sexes. Results The results of D-dimer level in men and women of the same age group had no statistically significant difference （t=0. 372-1. 092,P〉0.05）. There was no statistically significant difference （t=0. 954,P〉0.05） in groups of 18 to 49 and 50-59 and 〉60 years old group was compared to the other two groups,the concentration of plasma D-dimer between had statistically significant difference （t= 5. 972, P〈0.05）. The normal reference range of D-dimer in people of 18 to 59 was 0-225 mg/L,and 〉60 was 0-297 mg/L. Conclusion The concentrations of plasma D-dimer level has nothing to do with sex,and is associated with age,along with the age increase D-dimer level is on the rise. Different people,different in- struments using auxiliary reagents should establish its own normal reference range.