前列腺癌DU145细胞相关蛋白在构建体内三维空间模型中的表达研究

A comparative study of MMP9,Fn1and Laminin protein expression in prostate cancer cells with constructing scaffolds

目的探讨前列腺癌相关蛋白在胶原、琼脂糖、基质胶、海藻酸钠加明胶4种支架材料培养的前列腺癌DU145细胞中的表达水平。方法将前列腺癌DU145细胞按照一定比例接种至胶原、琼脂糖、基质胶、海藻酸钠加明胶4种支架材料中,分别分组进行移植瘤模型的三维培养,然后采用免疫组化方法检测MMP9、FN1、Laminin等3个前列腺癌相关蛋白在3种支架材料中培养的DU145细胞构建移植瘤模型后培养的组织中表达情况。结果 MMP9在有支架材料培养的前列腺癌DU145中蛋白表达高于无支架材料培养的组织;FN1在海藻酸钠加明胶组中蛋白表达阴性,在琼脂糖与胶原组中蛋白表达阳性且明显强于空白组;Lamininm在空白组与海藻酸钠加明胶组中均表达阳性,但明显低于胶原、琼脂糖、基质胶组的强阳性。结论采用支架材料的三维培养体系优于无支架材料的二维培养体系。胶原为体内前列腺癌DU145细胞三维培养的最优支架材料。

Objective Discussion of associated protein expression level in prostate cancer Du145 cell lines,which cultured individually in four kinds of scaffold materials, such as collagen,agarose, matrigel,alginate mixing gelatin. Methods Du145 cells was cultured in four kinds of Scaffold material groups,matrigel,collagen,agarose,alginate mixing gelatin,which transplant individually in the nude mouse. The expression of MMP9, Fnl and Laminin three kinds of proteins was determined by the immunohistochemistry （IHC） in four kinds of Scaffold material groups. Results Comparative the scaffold materials group and no-scaffold material groups,the MMP9 protein expression level was higher than without Scaffold material groups. FN1 protein was negative expression in alginate mixing ge- latin, the agarose group and collagen group was positive expression and significantly stronger than the control group. Lamininm protein was positive expression in the control group and alginate mixing gelatin group, but signifi- cantly lower than the collagengroup, agarose group, Matrigel group. Conclusion Three-dimensional cultured system is better than the two-dimensional cultured system. Collagen was the optimal Scaffold materials in vivo three-dimensional cultured prostate cancer Du145 cells.