摘要
目的研究1α,25-二羟维生素D_3(1α,25-(OH)_2D_3)对破骨细胞(osteoclast,OC)形成及骨吸收活性的影响。方法在巨噬细胞集落刺激因子(macrophage colony stimulating factor,M-CSF)和核因子-κB受体活化因子配体(receptor activator for nuclear factor-иB ligand,RANKL)诱导破骨细胞前体细胞RAW264.7细胞分化为OC的基础上,添加不同浓度1α,25-(OH)_2D_3(10^(-7),10^(-8)和10^(-9)mol/L),通过抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase,TRAP)染色鉴定成熟OC的形成,环境扫描电镜观察牛皮质骨片吸收陷窝。结果各浓度1α,25-(OH)_2D_3均可以促进RAW264.7细胞分化为OC,并增强其骨吸收活力。其中,10^(-8)mol/L的1α,25-(OH)_2D_3诱导效果最好,所形成的OC数最多,骨吸收活性最强。结论 1α,25-(OH)_2D_3能促进OC形成,增强骨吸收活性,从而调节骨代谢。
Objective To study the effects of la,25-dihydroxyvitamin D3 （1α,25-（OH）2D3） on osteoclastogenesis and bone re-absorption activity. Methods RAW264.7 cells were treated with different concentrations of 1α,25-（OH）2D3 （10^-7, 10^-8 and 10^-9 mol/L） under the induction of macrophage-colony stimulating factor （M-CSF） and receptor activator of nuclear factor-κB ligand （RANKL） in vitro respectively. The formation of osteoclasts was detected by tartrate resistant acid phosphatase （TRAP） staining. Analysis of cortical sclerite lacunar re-absorption was carried out using a scanning electron microscope. Results The treatment of 1α,25-（OH）2D3 increased the number of osteoclasts and enhanced bone re-absorption activity. This effect was most obvious at the concentration of 108 mol/L. Conclusion 1α,25-（OH）2D3 could regulate bone metabolism by promoting osteoclastogenesis and bone re-absorption.
出处
《营养学报》
CAS
CSCD
北大核心
2015年第6期607-610,613,共5页
Acta Nutrimenta Sinica
基金
National Natural Science Foundation of China(grant nos.31172373 and 31372495)
the National Science Foundation for Distinguished Young Scholars of China(grant no.31302154)
the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(grant no.13KJB230002)
the Project Funded by the Cultivate Middle-aged and Young Science Leaders of Colleges and Universities of Jiangsu Province(Su teachers [2010]27)
the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions
