SPF级FMMU白化豚鼠基础生理指标的测定与比较

The Measurement and Comparison of Basic Physiological Index of SPF FMMU Albimo Guinea-Pig

目的测定与比较SPF级FMMU白化豚鼠血液指标、心率、颈总动脉压及心室压、脏器参数。方法取30只雌雄各半、体质量250~300 g范围内SPF级FMMU白化豚鼠,心脏采血后分别检测血常规、血生化及血气指标;穿刺颈总动脉量取动脉压,辅助呼吸下开胸测定心室压;解剖后称量各脏器质量。结果雄性与雌性豚鼠比较：血液指标中,红细胞、血红蛋白、红细胞压积、谷草转氨酶、淀粉酶、胆固醇、二氧化碳总量、实际碳酸氢根、钠离子、总血红蛋白差异有显著性意义（P〈0.05或P〈0.01）;颈总动脉舒张压、颈总动脉平均压、左心室舒张压、右心室收缩压、右心室平均压差异有显著性意义（P〈0.05或P〈0.01）;体质量、脑质量差异以及脑垂体、肾上腺、左肾、右肾脏器系数差异有显著性意义（P〈0.05或P〈0.01）。结论不同性别的SPF级FMMU白化豚鼠血生理生化、血气、颈总动脉压、心室压指标及脏器参数存在差异。

Objective To determine and compare the basic parameters of blood,heart rate,carotid artery pressure,ventricular pressure and organ parameters of SPF（ specific pathogen free） FMMU albino guinea-pig. Method The heart blood was collected and blood physiological and biochemical index were measured in a total of 30 SPF FMMU albino guinea-pigs of about 250—300 g weight,and blood gas was determined. Blood pressure in carotid artery was measured by arterial puncture. Ventricular pressure and heart rate were measured on the respiratory support condition. Result The differences of RBC、HGB、HCT、AST、AMY、CHOI、TCO2、HCO-3、THbc、Na＋in male and female SPF FMMU albino guinea-pig were significant（ P〈0. 05 or P〈0. 01）. The differences of carotid diastolic blood pressure and mean arterial pressure,left ventricular diastolic pressure,right ventricular systolic pressure and mean pressure were also significant（ P〈0. 05 or P〈0. 01）. Finally,differences in both body weight and brain weight,and organ coefficient of pituitary,adrenal gland and left / right kidney were significantly different（ P〈0. 05 or P〈0. 01）. Conclusion There were differences of blood physiology and biochemistry,blood gas,carotid artery pressure,ventricular pressure and organ parameter in different gender of the SPF FMMU albino guinea-pig.