不同浓度生物活性玻璃对早期釉质龋再矿化的作用

The effects of bioactive glass of different concentrations on the remineralization of the enamel with early caries

目前:探讨不同浓度生物活性玻璃促进早期釉质龋再矿化的效果。方法:收集新鲜拔除的牛切牙,制备釉质标本,随机分为显微硬度组(n=15)和能谱分析组(n=25)。各组标本在37℃人工脱矿液中脱矿72 h,然后用p H循环法模拟人的口腔环境,将标本分别浸泡在30、60、90 g/L生物活性玻璃溶液内,2次/d,5 min/次,循环15 d。用显微硬度仪在釉质块脱矿前及再矿化后分别测量其表面显微硬度,能谱分析仪对釉质表面的元素进行分析。结果:脱矿处理后各组样本显微硬度均明显降底,生物活性玻璃溶液处理后显微硬度均明显提高,其中60 g/L组的显微硬度差值最高,30 g/L组最低(P﹤0.05)。钙磷比以90 g/L组最高,60 g/L组次之。结论:60 g/L生物活性玻璃促进早期釉质龋再矿化的疗效较好,90 g/L生物活性玻璃抗酸性最强。

AIM： To evaluate the effects of different concentrations of bioactive glass（ BG） on remineralization of the enamel with early caries. METHODS： Freshly extracted bovine incisors were selected and used for enamel specimen preparation. After preparation,the specimens were placed in demineralization solution at 37 ℃ for 72 h to establish early enamel caries. Then p H cycle method was used for remineralization using 30,60 and 90 g / L of bioactive glass respectively. After 15 d of p H cylcle,enamel micro hardness（ MH） was measured by micro hardness tester（ n = 15）,and the elements of the enamel were analyzed by X-ray energy spectrometer（ EDX）（ n = 25）. RESLUTS：After demineralization the MH of all the samples was decreased（ P〈0. 05）. After BG treatment the MH was increased（ P〈0. 05）,in 60 g/L group was the highest followed by that in 90 g/L group and 30 g/L group respectively（ P〈0. 05）. The molar ratio of Ca and P in 90 g/L group was the highest,followed by 60 g/L group（ P ﹤ 0. 05）.CONCLUTION： 60 g / L BG is most effective in remineralization of the enamel with early caries. 90 g / L BG is most resistant to the acid challenge.