HPLC法同时测定参芪颗粒中3种异黄酮成分的含量

Simultaneous Determination of 3 Isoflavones in Shenqi Granule by HPLC

目的：建立同时测定参芪颗粒中毛蕊异黄酮葡萄糖苷、毛蕊异黄酮、芒柄花素含量的方法.方法：采用高效液相色谱法.色谱柱为Welch Ultimate XB-C18,流动相为乙腈-0.2%甲酸溶液（梯度洗脱）,检测波长为250 nm,柱温为35℃,流速为1.0 ml/min,进样量为10μl.结果：毛蕊异黄酮葡萄糖苷、毛蕊异黄酮、芒柄花素进样量线性范围分别为0.008～0.32、0.005～0.20、0.007～0.28μg（r为0.999 9、0.999 7、0.999 8）;精密度、稳定性、重复性试验的RSD≤1.59%;加样回收率范围分别为98.17%～99.71%、98.03%～99.66%、98.16%～99.12%,RSD分别为0.67%、0.57%、0.43%（n=6）.结论：该方法简便、准确、可靠,可用于参芪颗粒中黄酮类成分的含量测定.

OBJECTIVE：To establish a method for simultaneous determination of the confents of calycosin-7-glucoside,calyco-sin and formononetin in Shenqi granule. METHODS：HPLC was performed on the column of Welch Ultimate XB-C18 with mobile phase of acetonitrile-0.2% Formic acid solution （gradient elution） at flow rate of 1.0 ml/min,detection wavelength was 250 nm, column temperature was 35 ℃,and injection volume was 10 μl. RESULTS：The linear range of sample quantity was 0.008-0.32 μg for calycosin-7-glucoside（r=0.999 9）,0.005-0.20 μg for calycosin（r=0.999 7）and 0.007-0.28 μg for formononetin（r=0.999 8）;RSDs of precsion,stability and reproducibility tests were no more than 1.59%;recoveries were 98.17%-99.17%（RSD=0.67%, n=6）,98.03%-99.66%（RSD=0.57%,n=6） and 98.16%-99.12%（RSD=0.43%,n=6）. CONCLUSIONS：The method is sim-ple,accurate and reliable,and can be used for the content determination of Shenqi granule ofiso flavones.