畲药大发散的黄酮类化学成分研究

Study on the Flavonoids in She Medicine Eupatorium chinense

目的：研究畲药大发散中的黄酮类成分.方法：采用硅胶、ODS、Sephadex LH-20柱色谱对畲药大发散中黄酮类成分进行分离纯化,根据理化性质和波谱数据分析鉴定化合物结构.结果：从大发散的乙酸乙酯萃取部位中共分离得到10个黄酮类成分,分别鉴定为苜蓿素（1）、槲皮素（2）、山柰酚（3）、木犀草素（4）、木犀草素-7-O-β-D-葡萄糖苷（5）、4-甲氧基苜蓿素（6）、槲皮素-3-O-β-D-葡萄糖苷（7）、山柰酚-3-O-β-D-葡萄糖苷（8）、山柰酚-3-O-芸香糖苷（9）和芦丁（10）.结论：化合物1～10均为首次从畲药大发散中分离得到,本研究为大发散质量评价奠定了一定基础.

OBJECTIVE：To study the flavonoids in She medicine Eupatorium chinense. METHODS：Silica gel,ODS and Sep-hadex LH-20 column chromatography were conducted to isolate and purify the flavonoids in She medicine E. chinense,and com-pound structures were analyzed and identified based on the physicochemical properties and spectral data. RESULTS：From the ethyl acetate extract of E. chinense,10 flavonoids were isolated as tricin （1）,quercetin（2）,kaempferol（3）,luteolin（4）,luteo-lin-7-O-β-D-glucoside （5）,4-methoxyctricin （6）,quercetin-3-O-β-D-glucoside（7）,kaempferol-3-O-β-D-glucoside（8）,kaempfer-ol-3-O-rutinoside（9）and rutin（10）. CONCLUSIONS：Compound 1-10 are isolated from E. chinense for the first time. The study provides certain basis for the quality evaluation of E. chinense.