针刺对豚鼠肝外胆道系统运动功能的影响

Effect of Acupuncture on Motor Function of Extrahepatic Biliary System of Guinea Pig

目的:通过观察针刺"期门"、"天枢"、"阳陵泉"与耳部"胰胆"穴(耳迷走神经分布密集区)对豚鼠胆囊压力和Oddi括约肌运动功能的影响,探讨针刺不同穴位调节肝外胆道系统运动功能的效应。方法:选用成年豚鼠20只,雌雄不限,每组10只,分成两组分别进行Oddi括约肌的肌电与胆囊压力检测。本实验采用自身前后对照法,观察针刺前后Oddi括约肌肌电与胆囊压力变化,Oddi括约肌的肌电检测采用双钩电极埋植技术,胆囊压力检测采用胆囊内置小型人工球囊技术。豚鼠麻醉后,分别记录正常豚鼠的胆囊压力变化和Oddi括约肌的肌电频率与幅值,待豚鼠状态稳定以后,针刺"期门"、"天枢"、"阳陵泉"与耳部"胰胆"穴1 min,观察针刺上述穴位对豚鼠胆囊压力和Oddi括约肌肌电的影响。结果:针刺"期门"、"天枢"穴后,Oddi括约肌肌电频率与针刺前相比均明显增多(频率:P<0.01,幅值P<0.05),胆囊压力幅值明显下降(P<0.05);针刺耳部"胰胆"和"阳陵泉"穴后,Oddi括约肌肌电频率和幅值与针刺前相比均明显下降(P<0.05),胆囊压力幅值明显上升(P<0.01)。结论:针刺"期门"、"天枢"穴能够明显抑制胆囊的运动、促进Oddi括约肌的肌电释放;针刺耳部"胰胆"和"阳陵泉"穴能够显著促进胆囊的收缩运动、抑制Oddi括约肌的肌电释放。

This study was aimed to compare the effects of acupuncture stimulation on different acupoints, which included LR14-Qimen, ST25-Tianshu, GB34-Yanglingquan and CO11-pancreas and gallbladder（auricular vagus nerve intensively distributed areas） on gallbladder pressure and Oddi sphincter function of guinea pig, in order to discuss acupuncture effect on different acupoints in the regulation of motor function of extrahepatic biliary system. A total of 20 adult guinea pigs of both sexes were randomly divided into two groups, with 10 in each group. The detection was made on changes of Oddi sphincter electromyography and gallbladder pressure before and after acupuncture. A double-hook electrode was implanted into the Oddi sphincter for the detection of Oddi sphincter electromyography. And one small artificial balloon was put into the gallbladder to measure the intrabiliary pressure. After the guinea pig was anesthetized, changes of gallbladder pressure as well as the electromyography frequency and amplitude index of Oddi sphincter of the normal guinea pig were recorded. When the guinea pig was stabilized, acupuncture was given on LR14, ST25, GB34 and CO11 for 1 min. Observations were made on acupuncture effects of gallbladder pressure and Oddi sphincter function of guinea pig. The results showed that after acupuncture on LR14 and ST25, the electromyography frequency of Oddi sphincter was obviously increased compared with before acupuncture（frequency： P〈0.01, amplitude： P〈0.05）; the gallbladder pressure was obviously decreased（P〈0.05）. After acupuncture on CO11 and GB34, the electromyography frequency and amplitude index of Oddi sphincter were obviously decreased compared with before acupuncture（P〈0.05）; the gallbladder pressure was obviously increased（P〈0.01）. It was concluded that acupuncture on LR14 and ST25 can obviously inhibit the movement of gallbladder and promote the myoelectricity release of Oddi sphincter. Acupuncturing on CO11 and GB34 can apparently promote the shrinking movement of gallbladder and inhibit the myoelectricity release of Oddi sphincter.