丙泊酚和依托咪酯对脓毒症小鼠炎症反应与氧化应激的影响

Effects of propofol and etomidate on inflammation and oxidative stress in septic mice

目的观察丙泊酚及依托咪酯对脓毒症小鼠炎症反应与氧化应激的影响。方法雄性成年BALB/c小鼠64只,随机分为四组(n=16):正常对照组(N组)、脓毒症组(S组)、丙泊酚处理组(P组)及依托咪酯处理组(E组)。脂多糖(LPS,20mg/kg)腹腔注射建立脓毒症模型,丙泊酚(60mg/kg)或依托咪酯(10mg/kg)于LPS注射后0.5h腹腔注射给药。于LPS注射后2h及6h检测血清IL-6、IL-10浓度,6h检测肺、肝、肾组织中的丙二醛(MDA)含量。结果与N组比较,S组2h及6h血清IL-6浓度明显上升(P<0.05),IL-10浓度明显下降(P<0.05);6h肺、肝、肾组织MDA含量均明显上升(P<0.05)。与S组比较,P、E两组2h及6h血清IL-6浓度均明显下降(P<0.05),IL-10浓度均明显上升(P<0.05);E组6h肺、肝、肾组织MDA含量明显下降(P<0.05),P组6h仅肺组织MDA含量明显下降(P<0.05)。E组2h血清IL-6浓度明显低于P组(P<0.05),2h及6h血清IL-10浓度明显高于P组(P<0.05);6h肺、肝、肾组织MDA含量均明显低于P组(P<0.05)。结论丙泊酚和依托咪酯腹腔注射均可减轻LPS所致脓毒症小鼠炎症反应及氧化应激损伤,且依托咪酯的作用更强。

Objective To observe the effects of propofol and etomidate on inflammation and ox-idative stress in septic mice.Methods Sixty-four male adult BALB/c mice were randomly divided into four groups：normal control group （N）,sepsis group （S）,propofol treatment group （P）and etomid-ate treatment group （E）.The septic mice model was established by lipopolysaccharide （LPS,20 mg/kg）intraperitoneal injection,and propofol （60 mg/kg）or etomidate （10 mg/kg）was injected in the abdominal cavity at 0.5 h after LPS injection.Serum interleukin-6 （IL-6）concentrations and in-terleukin-10 （IL-10）concentrations were measured at 2 h and 6 h after LPS injection;malondialde-hyde （MDA）content of lung,liver and kidney tissue was measured at 6 h after LPS injection. Results Compared with group N,serum IL-6 concentrations increased significantly （P 〈0.05）,and IL-10 concentrations decreased significantly （P 〈0.05）at 2 h and 6 h after LPS injection in group S;MDA content of lung,liver,kidney increased significantly （P 〈0.05 ）at 6 h after LPS injection in group S;Compared with group S,serum IL-6 concentrations decreased significantly （P 〈0.05）,and IL-10 concentrations increased significantly （P 〈 0.05 ）at 2 h and 6 h after LPS injection in both group P and E;MDA content of lung,liver,kidney decreased significantly （P 〈0.05 ）at 6 h after LPS injection in group E,but only MDA content of lung decreased significantly （P 〈0.05）at 6 h af-ter LPS injection in group P;Compared with group P,serum IL-6 concentrations was significantly lower （P 〈0.05）,and IL-10 concentrations was significantly higher （P 〈0.05）at 2 h and 6 h after LPS injection in group E;MDA content of lung,liver,kidney was significantly lower （P 〈0.05）at 6 h after LPS injection in group E.Conclusion Both propofol and etomidate injected in the abdominal cavity can reduce injury of inflammatory and oxidative stress in septic mice induced by LPS,and the effect of etomidate is more significant.