丙泊酚对老年大鼠脑颞叶GABAR1和NMDAR2B表达的影响

The expression of GABAR1 and NMDAR2B in aged rat cerebral temporal lobe by propofol injection

目的观察腹腔注射丙泊酚后老年大鼠学习记忆变化以及脑左侧颞叶γ-氨基丁酸受体1(GABAR1)和NMDA受体2B(NMDAR2B)表达的变化。方法雄性SD老年大鼠随机分为三组,每组20只。P1和P7组腹腔注射丙泊酚50mg/kg,维持麻醉3h,C组给予相同剂量的乳剂。分别在给药后第1、7天进行Morris水迷宫实验,测试结束后采集大鼠左侧脑颞叶,采用FISH技术和免疫荧光检测GABAR1和NMDAR2B在基因转录以及蛋白表达的变化。结果与C组比较,P1组潜伏时间明显延长,穿越目标次数明显减少(P<0.05)。P1组GABAR1mRNA转录和蛋白表达明显增多(P<0.05),NMDAR2BmRNA转录和蛋白表达明显减少(P<0.05)。结论大鼠在使用丙泊酚后,其大脑的短时记忆受到影响,这种影响可能与大脑颞叶的功能有关,而机制可能与颞叶区域NMDAR2B表达下调而同时出现GABAR1表达上调有关。

Objective To observe the change of learning and memory quality and the expression change of GABAR1 and NMDAR2B in aged SD rat cerebral temporal lobe by propofol injection and discuss the influence that may be caused by propofol.Methods Aged male sd rats were randomly divided into three groups,each group of 20.Propofol intraperitoneal injection was implemented to groups P1 and P7 according 50 mg/kg and maintained anesthesia for 3 hours.The same dose of emulsion was given to control group. Morris water maze test was done in the first days and the seventh days after intraperitoneal injection.Then the left temporal lobe was gathered and the mRNA transcription and protein expression of GABAR1 and NMDAR2B were detected by FISH and Immunofluorescence technique.Results Compared with control group,the latent time of group P1 was significantly prolonged and the numbers of passing through the target frequency were decreased significantly （P 〈0.05）.Group P7 had no significant differences.The mRNA transcription and protein expression of GABAR1 in group P1 were increased significantly and the mRNA transcription and protein expression of NMDAR2B in group P1 were decreased significantly （P 〈0.05 ）. Group P7 had no significant differences.Conclusion The short-term memory of brain declined after propofol was used.This may be related to the function of temporal lobe.The mechanism may be related to the up regulation of GABAR1 and the down regulation of NMDAR2B simultaneously.