电针对帕金森病模型大鼠纹状体Glu浓度、GLT-1mRNA和GSmRNA表达的影响

Influence of electroacupuncture on Glu concentration,GLT-1mRNA and GSmRNA in the striatum of Parkinson's disease rats

目的探讨电针治疗帕金森病(PD)的作用机制。方法 50只雄性SD大鼠随机分为正常组、假手术组每组10只,模型组、电针组每组15只。PD大鼠旋转模型制备采用6-OHDA单侧纹状体立体定向微量注射法。假手术组注射方法和部位同模型组,仅注射含0.2%Vit C的生理盐水。电针组在模型制作成功后给予电针"风府、太冲"穴治疗,每天一次,每次30 min,7天为1疗程,连续治疗2个疗程。各组大鼠选取10只进行取材及处理。HPLC荧光法检测纹状体谷氨酸(Glu)浓度,RT-PCR法检测谷氨酸转运体-1(GLT-1mRNA)及谷氨酰胺合成酶(GSmRNA)蛋白活性的表达。结果电针组大鼠治疗前后旋转行为差异显著(P<0.01)。与正常组及假手术组比较,模型组大鼠Glu浓度显著升高,GLT-1mRNA与GSmRNA表达水平均显著降低(P<0.01);与模型组比较,电针组大鼠Glu浓度降低,GLT-1mRNA与GSmRNA表达水平均升高(P<0.05)。结论电针提高了脑内GLT-1mRNA与GSmRNA蛋白活性的表达,减轻了Glu引起的细胞毒作用,从而对PD多巴胺能神经元起到了一定的保护作用。

Objective To investigate the mechanism of acupuncture treatment of Parkinson＇s disease. Methods Fifty male SD rats were randomly divided into normal group,sham-operation group（ n = 10 / group）,model group,electroacupuncture（ EA） group（ n = 15 /group）. Rotary PD rat model was prepared by unilateral stereotactic microinjection of 6-OHDA in the striatum. In sham-operation group,the injection method and injection site was same with model group,but the injection was replaced with 0. 2%Vit C of physiological saline. The EA group was treated for 30 min by electroacupuncture at ＂ Fengfu＂（ GV16）, ＂ Taichong＂（ LR3）after the model successfully made,once a day for two courses,7 days for a course of treatment. Select 10 rats in each group for materials and processing. Glu concentration in the striatum was detected by HPLC assay. RT-PCR method was used to detect the expression of GLT-1mRNA and the expression of GSmRNA. Results Compared with the normal group and Sham-operation group,Glu concentration in model group significantly increased,both of GLT-1mRNA expression and GSmRNA expression in model group were significantly decreased（ P〈0. 01）. Compared with the model group,Glu concentration in EA group obviously decreased,both of GLT-1mRNA expression and GSmRNA expression in EA group were increased（ P〈0. 05）. Conclusion-Electroacupuncture therapy can improve the brain glutamate transporter GLT-1 expression and GS expression activity,reduce the cytotoxicity caused by the extracellular Glu relevant. Thus EA for dopaminergic neurons of PD plays a part of protective role.